{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:54:46Z","timestamp":1787237686172,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T00:00:00Z","timestamp":1523318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      To clarify the characteristic growth of melanocytes (MCs) and Keratinocytes (KCs) in vitro and discuss the mechanism of culturing autologous melanocytes in the treatment of\n                      <jats:italic>vitiligo<\/jats:italic>\n                      . Epidermis cells derived from normal skin tissues were isolated and cultured in vitro. Melanocytes in DOPA staining were observed. The expression level of markers in MCs was detected by qRT\u2010PCR and the percentage of MCs and KCs were detected by flow cytometry. Cells derived from normal skin tissues mainly included KCs, MCs, and fibroblasts. There were significant differences between the percentage of KC, MC, fibroblasts (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &lt;\u20090.05), and the expression of Microphthalmia\u2010associated transcription factor (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &lt;\u20090.05) and Tyrosinase\u2010related protein\u20102 (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &lt;\u20090.05) in the second, 10th, 20th, and 30th day. Significant differences were also found between the average numbers of MC stained by DOPA (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &lt;\u20090.05) and the average percentage of MCs in the 10th, 20th, and 30th Day (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &lt;\u20090.05). But there were no significant differences between the average percentage of KCs in the 10th, 20th, and 30th Day (\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &gt;\u20090.05) detected by flow cytometry. The number of MCs co\u2010cultured with KCs in vitro reached the maximum in the 20th Day and this co\u2010cultured model may contribute to the growth of MCs which could be used in the treatment of\n                      <jats:italic>vitiligo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.26825","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T20:50:00Z","timestamp":1523393400000},"page":"6173-6180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A preliminary study of growth characteristics of melanocytes co\u2010cultured with keratinocytes in vitro"],"prefix":"10.1002","volume":"119","author":[{"given":"Chengzhong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Dermatology Shanghai General Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Dermatology Wuxi Branch of Ruijin Hospital Shanghai Jiaotong University School of Medicine Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai General Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyu","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai General Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouwei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai General Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0401-1175","authenticated-orcid":false,"given":"Weimin","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Shanghai General Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.293.6.730"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","unstructured":"CzajkowskiR PlacekW DrewaT KowaliszynB SirJ WeissW. 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