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We previously reported that PTH stimulates the expression of matrix metalloproteinase\u201013 (MMP\u201013) in rat osteoblasts and that MMP\u201013 plays a key role in bone remodeling, endochondral bone formation, and bone repair. Recent evidence indicated that microRNAs (miRNAs) have regulatory functions in bone metabolism. In this study, we hypothesized that the down\u2010regulation of miRNAs that target MMP\u201013 by PTH leads to the stimulation of MMP\u201013 expression in osteoblasts. We used various bioinformatic tools to identify miRNAs that putatively target rat MMP\u201013. Among these miRNAs, the expression of miR\u2010532\u20105p in rat osteoblasts decreased at 4\u2009h of PTH\u2010treatment, whereas MMP\u201013 mRNA expression was maximal at the same time point. When an miR\u2010532\u20105p mimic was transiently transfected into UMR\u2010106\u201001 cells, MMP\u201013 mRNA and protein expression decreased. Using a luciferase reporter assay system, we also identified that miR\u2010532\u20105p directly targeted the 3\u2032 UTRs of MMP\u201013 gene. Based on these results, we suggest that PTH\u2010induced down\u2010regulation of miR\u2010532\u20105p resulted in the stimulation of MMP\u201013 expression in rat osteoblasts. This study identified a significant role of miRNA in controlling bone remodeling via PTH\u2010stimulated MMP\u201013 expression. This finding enhances our understanding of bone metabolism and bone\u2010related diseases and it could provide information regarding the usage of miRNAs as therapeutic agents or biomarkers.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26827","type":"journal-article","created":{"date-parts":[[2018,4,7]],"date-time":"2018-04-07T02:43:43Z","timestamp":1523069023000},"page":"6181-6193","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Parathyroid hormone\u2010induced down\u2010regulation of miR\u2010532\u20105p for matrix metalloproteinase\u201013 expression in rat osteoblasts"],"prefix":"10.1002","volume":"119","author":[{"given":"Vishal","family":"Mohanakrishnan","sequence":"first","affiliation":[{"name":"Department of Biotechnology School of Bioengineering SRM Institute of Science and Technology Kattankulathur Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arumugam","family":"Balasubramanian","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Bioengineering SRM Institute of Science and Technology Kattankulathur Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gokulnath","family":"Mahalingam","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Bioengineering SRM Institute of Science and Technology Kattankulathur Tamil Nadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola Chennell","family":"Partridge","sequence":"additional","affiliation":[{"name":"Department of Basic Science and Craniofacial Biology New York University College of Dentistry New York University New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilangovan","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Dr. A.L.M. 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