{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:03:33Z","timestamp":1770746613582,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T00:00:00Z","timestamp":1525996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["92\u201001\u201030\u201021632"],"award-info":[{"award-number":["92\u201001\u201030\u201021632"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>AR\u2010mediated androgen signaling plays a key role in female reproductive system. Granulosa\u2010lutein cells (GCs) are the main sites for expression of androgen receptor (AR). There is also a close relation between AKT signaling and AR. Here, we assayed the role for a synthetic AR ligand methyltrienolone (R1881) in expressions of AKTs and AR. Controlled ovarian hyperstimulation (COH) was performed in 20 normal women. Mural GCs were isolated by filtration method, cultured, and passaged. Then, the cells were starved for 48\u2009h with 10% charcoal stripped FBS. The cells were then treated with R1881, bicalutamide (AR blocker), LY294002 (PI3K\/AKT pathway blocker), and combination of them for 48\u2009h. Finally, GCs were evaluated for quantitative real\u2010time PCR analysis of AKT1, AKT2, AKT3, and AR, and also Western blot assessment of total AKT and phosphorylated AKT (p\u2010AKT) [Ser473 and Thr308]. Addition of R1881 to the GCs culture showed high expressions of AKT1, AKT2, and AKT3 (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.05 vs LY294002 group and bicalutamide group). Expressions of AKT1 and AKT2 were decreased in the GCs under exposure to bicalutamide or LY294002 (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.05 vs R1881). AKT1, AKT2, and AKT3 showed decreased rates of expressions in the LY294002\u2009+\u2009bicalutamide group (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.05 vs R1881). AR, total AKT and p\u2010AKT showed no significant differences between groups. Our findings indicate that 46\u2009h exposure with R1881 could affect AKTs expressions in the GCs of pre\u2010ovulatory phase, but it cannot promote AR expression and AKTs activation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26861","type":"journal-article","created":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T12:21:16Z","timestamp":1526041276000},"page":"7204-7211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Roles of methyltrienolone (R1881) in AKTs and AR expression patterns of cultured granulosa\u2010lutein cells"],"prefix":"10.1002","volume":"119","author":[{"given":"Saeid","family":"Nekoonam","sequence":"first","affiliation":[{"name":"Department of Anatomy, School of Medicine Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Naji","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine Tehran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2004-3465","authenticated-orcid":false,"given":"Keywan","family":"Mortezaee","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine Kurdistan University of Medical Sciences Sanandaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fardin","family":"Amidi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, School of Medicine Tehran University of Medical Sciences Tehran Iran"},{"name":"Department of Infertility, Shariati Hospital Tehran University of Medical Sciences Tehran 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