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TGF\u2010\u03b22 has been implicated in this fibrosis. Previous studies have focused on the role of hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) in fibrotic diseases, but the role of HIF\u20101\u03b1 in the TGF\u2010\u03b22\u2010mediated fibrosis in HLE is not known. TGF\u2010\u03b22 treatment (10\u2009ng\/mL, 48\u2009h) increased the HIF\u20101\u03b1 levels along with the EMT markers in cultured human lens epithelial cells (FHL124 cells). The increase in HIF\u20101\u03b1 corresponded to an increase in VEGF\u2010A in the culture medium. However, exogenous addition of VEGF\u2010A (up to 10\u2009ng\/mL) did not alter the EMT marker levels in HLE. Addition of a prolyl hydroxylase inhibitor, dimethyloxalylglycine (DMOG, up to 10\u2009\u00b5M), enhanced the levels of HIF\u20101\u03b1, and secreted VEGF\u2010A but did not alter the EMT marker levels. However, treatment of cells with a HIF\u20101\u03b1 translational inhibitor, KC7F2, significantly reduced the TGF\u2010\u03b22\u2010mediated EMT response. This was accompanied by a reduction in the ERK phosphorylation and nuclear translocation of Snail and Slug. Together, these data suggest that HIF\u20101\u03b1 is important for the TGF\u2010\u03b22\u2010mediated EMT of human lens epithelial cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.26877","type":"journal-article","created":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T11:13:41Z","timestamp":1524654821000},"page":"6814-6827","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The role of HIF\u20101\u03b1 in the TGF\u2010\u03b22\u2010mediated epithelial\u2010to\u2010mesenchymal transition of human lens epithelial cells"],"prefix":"10.1002","volume":"119","author":[{"given":"Rooban B.","family":"Nahomi","sequence":"first","affiliation":[{"name":"Department of Ophthalmology University of Colorado Aurora Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4737-8847","authenticated-orcid":false,"given":"Ram 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