{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:22:46Z","timestamp":1792293766921,"version":"4.4.0"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T00:00:00Z","timestamp":1526342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>The response to glucagon and adrenaline in cancer cachexia is poorly known. The aim of this study was to investigate the response to glucagon, adrenergic agonists (\u03b1 and \u03b2) and cyclic adenosine monophosphate (cAMP) on glycogenolysis, gluconeogenesis, and glycolysis in liver perfusion of Walker\u2010256 tumor\u2010bearing rats with advanced cachexia. Liver ATP content was also investigated. Rats without tumor (healthy) were used as controls. Agonists \u03b1 (phenylephrine) and \u03b2 (isoproterenol) adrenergic, instead of adrenaline, and cAMP, the second messenger of glucagon and isoproterenol, were used in an attempt to identify mechanisms involved in the responses. Glucagon (1\u2009nM) stimulated glycogenolysis and gluconeogenesis and inhibited glycolysis in the liver of healthy and tumor\u2010bearing rats, but their effects were lower in tumor\u2010bearing rats. Isoproterenol (20\u2009\u00b5M) stimulated glycogenolysis, gluconeogenesis, and glycolysis in healthy rats and had virtually no effect in tumor\u2010bearing rats. cAMP (9\u2009\u00b5M) also stimulated glycogenolysis and gluconeogenesis and inhibited glycolysis in healthy rats but had practically no effect in tumor\u2010bearing rats. Phenylephrine (2\u2009\u00b5M) stimulated glycogenolysis and gluconeogenesis and inhibited glycolysis and these effects were also lower in tumor\u2010bearing rats than in healthy. Liver ATP content was lower in tumor\u2010bearing rats. In conclusion, tumor\u2010bearing rats with advanced cachexia showed a decreased hepatic response to glucagon, adrenergic agonists (\u03b1 and \u03b2), and cAMP in glycogenolysis, gluconeogenesis, and glycolysis, which may be due to a reduced rate of regulatory enzyme phosphorylation caused by the low ATP levels in the liver.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27027","type":"journal-article","created":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T12:05:33Z","timestamp":1526385933000},"page":"7300-7309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Decreased hepatic response to glucagon, adrenergic agonists, and cAMP in glycogenolysis, gluconeogenesis, and glycolysis in tumor\u2010bearing rats"],"prefix":"10.1002","volume":"119","author":[{"given":"Giuliana R.","family":"Biazi","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina, PR 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