{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T14:59:16Z","timestamp":1781708356804,"version":"3.54.5"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T00:00:00Z","timestamp":1526601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Post kala\u2010azar dermal leishmaniasis (PKDL) is often considered to be the anthroponotic reservoir of visceral leishmaniasis (VL) in India. A better understanding of the host immune\u2010response in dermal lesions of PKDL patients is therefore of utmost significance to minimize such patients and to restrict VL transmission. Although the innate immune response is known to play an important role in parasite clearance from dermal lesions, the actual contribution of innate cells to the pathogenicity of PKDL is poorly understood. The present study explored the immune\u2010pathogenesis of PKDL patients to understand the expression of CD62L, CD11b, CXCL8\/IL\u20108, and MIP1\u2010\u03b1 and their contribution in signaling during innate cell trafficking. Twenty\u2010five individuals were enrolled, who comprised eight active and untreated macular cases, seven active and untreated cases with papulo\u2010nodular PKDL manifestations, five successfully treated post PKDL cases and five healthy individuals from a non\u2010endemic region of Bihar, India. The immunological investigation was performed on biopsy specimens prepared with a disaggregation technique and blood samples. We observed that the PMNs in nodular patients displayed decreased L\u2010selectin (CD62L) levels and increased integrin (CD11b) expression compared with those in macular patients. Further analysis showed that lower PMN extravasation in macular patients occurred because of inadequate CXCL8\/ IL\u20108 release. In summary, <jats:italic>Leishmania donovani<\/jats:italic> (<jats:italic>L. donovani<\/jats:italic>) infection in macular PKDL patients decreased leucocyte rolling (L\u2010selectin shedding) and induced up\u2010regulation of the cellular signaling factors involved in pathogenesis (ERK1\/2) as well as down regulated the signaling elements (p38 MAPK) involved in the Th1 response, especially in PMNs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27048","type":"journal-article","created":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T13:06:03Z","timestamp":1526648763000},"page":"7406-7418","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cytokines and chemokines differentially regulate innate immune cell trafficking during post kala\u2010azar dermal leishmaniasis"],"prefix":"10.1002","volume":"119","author":[{"given":"Ashish K.","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Immunology Rajendra Memorial Research Institute of Medical Sciences Patna India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidya N. 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