{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:27:58Z","timestamp":1790857678012,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T00:00:00Z","timestamp":1531699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG6E0331"],"award-info":[{"award-number":["CMRPG6E0331"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012553","name":"Chang Gung Memorial Hospital","doi-asserted-by":"publisher","award":["CMRPG6E0332"],"award-info":[{"award-number":["CMRPG6E0332"]}],"id":[{"id":"10.13039\/100012553","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gastric cancer is the third leading cause of cancer mortality all over the world. The combination therapy of surgery with chemotherapy, that is, 5\u2010fluorouracil (5\u2010FU) and platinum\u2010containing anticancer drugs, is becoming a current clinical strategy for patients with gastric cancer because of the lower curative rate and higher cancer recurrence rate of patients treated with only surgery. However, the development of drug resistance in cancer cells is still the most challenge in clinical chemotherapy. Excision repair cross\u2010complementing 1 (ERCC1), an essential member of nucleotide excision repair system, recently has been suggested to be a predictive biomarker of treatment evaluation and might affect the outcomes of chemotherapy. Thus, this study was aimed to investigate whether ERCC1 expression could be regulated, and its role in gastric cancer cells treated with 5\u2010FU and the underlying mechanism. Human AGS gastric cancer cells were used in this study. It was shown that ERCC1 expression could be upregulated in AGS cells treated with 5\u2010FU and this upregulation could subsequently attenuate the cytotoxicity of 5\u2010FU in AGS cells. Moreover, 5\u2010FU\u2013upregulated ERCC1 expression was regulated by extracellular signal\u2010regulated kinase (ERK) 1\/2 and p38 signaling through activating the transcription factor c\u2010jun\/activator protein (AP)\u20101. These results indicated the role of ERCC1 in the development of drug resistance to 5\u2010FU in AGS cells. The mechanism elucidation concerning the ERK1\/2 and p38 kinases and transcription factor c\u2010jun\/AP\u20101 might contribute another idea to the development of chemotherapy strategy for the gastric cancers in the future.<\/jats:p>","DOI":"10.1002\/jcb.27073","type":"journal-article","created":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T13:43:56Z","timestamp":1531748636000},"page":"8472-8480","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effect of 5\u2010fluorouracil on excision repair cross\u2010complementing 1 expression and consequent cytotoxicity regulation in human gastric cancer cells"],"prefix":"10.1002","volume":"119","author":[{"given":"Jing\u2010Lan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pathology Chang Gung Memorial Hospital Chiayi Branch  Chiayi 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