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BALB\/c mice bearing TUBO carcinoma were subcutaneously immunized with formulations containing 10\u2009\u00b5g P5 peptide and 25\u2009\u00b5g MPL three times with 2\u2010week intervals. To determine immuno responses in immunized mice, the amount of released interferon\u2010\u03b3 and IL\u20104 were measured by the enzyme\u2010linked immunospot method and the flow cytometric analysis on the isolated splenocytes. The results demonstrated that tumor\u2010bearing mice immunized with Lip\/DOPE\/MPL\/P5 formulation had the most released interferon\u2010\u03b3 and the highest cytotoxic T lymphocyte responses that led to the lowest tumor size and the longest survival time than those of other formulations. The results achieved by Lip\/DOPE\/MPL\/P5 formulation could make it a suitable candidate to induce effective antigen\u2010specific tumor immunity against breast cancer.<\/jats:p>","DOI":"10.1002\/jcb.27090","type":"journal-article","created":{"date-parts":[[2018,10,31]],"date-time":"2018-10-31T00:49:17Z","timestamp":1540946957000},"page":"1294-1303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Preparation of nanoliposomes linked to HER2\/neu\u2010derived (P5) peptide containing MPL adjuvant as vaccine against breast cancer"],"prefix":"10.1002","volume":"120","author":[{"given":"Saeedeh","family":"Rastakhiz","sequence":"first","affiliation":[{"name":"Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences Mashhad Iran"},{"name":"Department of Pharmaceutical Nanotechnology School of Pharmacy Mashhad University of Medical Science Mashhad 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