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This study represents the use of the combinatorial immunoinformatics approach to develop a multiepitope subunit vaccine using the structural and nonstructural proteins of the Zika virus. The designed subunit vaccine consists of cytotoxic T\u2010lymphocyte\u00a0and helper T\u2010lymphocyte epitopes accompanied by suitable adjuvant and linkers. The presence of humoral immune response specific B\u2010cell epitopes was also confirmed by B\u2010cell epitope mapping among vaccine protein. Further, the vaccine protein was characterized for its allergenicity, antigenicity, and physiochemical parameters and found to be safe and immunogenic. Molecular docking and molecular dynamics studies of the vaccine protein with the toll\u2010like receptor\u20103 were performed to ensure the binding affinity and stability of their complex. Finally, in silico cloning was performed for the effective expression of vaccine construct in the microbial system (<jats:italic>Escherichia coli<\/jats:italic> K12 strain). Aforementioned approaches result in the multiepitope subunit vaccine which may have the ability to induce cellular as well as humoral immune response. Moreover, this study needs the experimental validation to prove the immunogenic and protective behavior of the developed subunit vaccine.<\/jats:p>","DOI":"10.1002\/jcb.27110","type":"journal-article","created":{"date-parts":[[2018,6,14]],"date-time":"2018-06-14T10:56:33Z","timestamp":1528973793000},"page":"7631-7642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":98,"title":["Designing B\u2010 and T\u2010cell multi\u2010epitope based subunit vaccine using immunoinformatics approach to control Zika virus infection"],"prefix":"10.1002","volume":"119","author":[{"given":"Rajan","family":"Kumar Pandey","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Life Sciences Central University of Rajasthan  Ajmer Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupal","family":"Ojha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Life 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