{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T14:41:50Z","timestamp":1754145710911,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T00:00:00Z","timestamp":1539475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hangzhou Municipal Science & Technology Commission","award":["20163501Y82"],"award-info":[{"award-number":["20163501Y82"]}]},{"name":"Science Technology Department of Zhejiang Province","award":["2017C33205"],"award-info":[{"award-number":["2017C33205"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies have revealed that long noncoding RNA (lncRNA) and microRNA\u00a0play a crucial role in autism, which is a childhood neurodevelopmental disorder with complicated genetic origins. Hence, the study concerns whether lncRNA C21orf121\/bone morphogenetic proteins 2 (BMP2)\/miR\u2010140\u20105p gene network affects directed differentiation of stem cells from human exfoliated deciduous teeth (SHED) to neuronal cells in rats with autism. Autism models were successfully established. The neuron cells that differentiated from SHED cell were identified. The expression of lncRNA C21orf121, miR\u2010140\u20105p, BMP2, Nestin, \u03b2III\u2010tubulin, and microtubule\u2010associated protein 2 (MAP2) and the expression of neuron\u2010specific enolase (NSE) were examined. Besides, the gap junction (GJ) function of SHED, the intracellular free Ca\n<jats:sup>2+<\/jats:sup> concentration, and the social behavior and repetitive stereotyped movements of rats in autism were detected. The target relationship between lncRNA C21orf121 and miR\u2010140\u20105p and that between miR\u2010140\u20105p and BMP2 were also verified. Firstly, we successfully isolated SHED and identified the differentiated neurons of SHED. Besides, the expression of BMP2, MAP2, Nestin, \u03b2III\u2010tubulin, NSE positive rate, GJ function, and intracellular free Ca\n<jats:sup>2+<\/jats:sup> concentration were increased with the upregulation of C21orf121 and downregulation of miR\u2010140\u20105p, and accumulated time of repetitive stereotyped movements decreased and the frequency of social behavior increased. The results indicate that lncRNA C21orf121 as a competing endogenous RNA\u00a0competes with BMP2 binding to miR\u2010140\u20105p, thereby promoting SHED to differentiate into neuronal cells via upregulating BMP2 expression.<\/jats:p>","DOI":"10.1002\/jcb.27313","type":"journal-article","created":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T08:47:00Z","timestamp":1539506820000},"page":"1464-1476","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Long noncoding RNA C21orf121\/bone morphogenetic protein 2\/microRNA\u2010140\u20105p gene network promotes directed differentiation of stem cells from human exfoliated deciduous teeth to neuronal cells"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3462-5602","authenticated-orcid":false,"given":"Jun","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Zhejiang Xiaoshan Hospital Hangzhou 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