{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:54:11Z","timestamp":1754398451023,"version":"3.40.4"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2015A030313431"],"award-info":[{"award-number":["2015A030313431"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81001284"],"award-info":[{"award-number":["81001284"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epithelial\u2010mesenchymal transformation of the medial edge epithelium is the most crucial process in embryonic palatal fusion. This study aimed to explore the relationship and potential mechanism between enhancer DNA methylation and mRNA expression of histone deacetylase 4 (HDAC4) during palatal fusion induced by maternal exposure to all\u2010trans retinoic acid (ATRA). Pregnant mice were administered ATRA (70\u2009mg\/kg) by gavage at embryonic gestation day 10.5 (E10.5) to establish a cleft palate (CP) model in C57BL\/6J mice. Control groups were given an equivalent volume of corn oil. Pregnant mice were dissected at E14.5 (n\u2009=\u20096) to obtain embryonic palates. HDAC4 enhancer DNA methylation data were obtained from a previous MethylRAD\u2010seq. Methylation\u2010specific polymerase chain reaction (MSP) and real\u2010time quantitative PCR were used to quantify enhancer methylation and the mRNA expression level of HDAC4. Enhancer DNA methylation at a non\u2010CpG site within the HDAC4 gene was hyper\u2010methylated at E14.5 (<jats:italic>P<\/jats:italic>: 0.011, log<jats:sub>2<\/jats:sub>FC:1.67). The MSP results indicated a similar trend, in agreement with the MethylRAD\u2010seq results. The change in the HDAC4 expression level was negatively correlated with its enhancer DNA methylation level, at the non\u2010CpG site, during palatal fusion induced by ATRA. Enhancer DNA methylation of HDAC4 might play an important regulatory role during palatogenesis, especially in embryonic palatal fusion at E 14.5, and may facilitate the development of novel epigenetic biomarkers in the treatment of CP.<\/jats:p>","DOI":"10.1002\/jcb.27320","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T06:56:06Z","timestamp":1535612166000},"page":"9967-9973","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Correlation between HDAC4 enhancer DNA methylation and mRNA expression during palatal fusion induced by all\u2010trans retinoic acid"],"prefix":"10.1002","volume":"119","author":[{"given":"Xuan","family":"Shu","sequence":"first","affiliation":[{"name":"Department of Burn and Plastic Surgery 2nd Affiliated Hospital of Shantou University Medical College  Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1752-628X","authenticated-orcid":false,"given":"Hongqiu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases 2nd Affiliated Hospital of Shantou University Medical College  Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenyou","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery 2nd Affiliated Hospital of Shantou University Medical College  Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijie","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery 2nd Affiliated Hospital of Shantou University Medical College  Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery 2nd Affiliated Hospital of Shantou University Medical College  Shantou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zejun","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery 2nd Affiliated Hospital of Shantou University Medical College  Shantou 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