{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T04:12:45Z","timestamp":1744863165381,"version":"3.40.4"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T00:00:00Z","timestamp":1534723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471975","81401683"],"award-info":[{"award-number":["81471975","81401683"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> excreted\u2010secreted antigens (ESA) could lead to the fetal abortion especially in the early stage of pregnancy. Deficit in regulatory T cells is a critical event in the fetal abortion. Transcription factor forkhead box p3 (Foxp3) mediates differentiation and functional roles on regulatory T cells. Previously, we revealed that ESA inhibited Foxp3 through the suppression of transforming growth factor\u2010\u03b2 type II receptor, phosphorylation of Smad2, Smad3, and Smad4. Knockdown of Smad2 collaborated with ESA to further inhibit Foxp3. The decrease in Foxp3 caused by ESA reversed via forced expression of Smad2, Smad3, and Smad4, respectively. In this study, we investigate whether other signaling pathways are implicated in ESA\u2010induced Foxp3 downregulation. EL4 cells were cultured and stimulated with ESA. Interleukin\u20102 receptor \u03b3 (IL\u20102R\u03b3) chain, Janus kinase 3 (JAK3), signal transducer and activator of transcription 5 (Stat5), Stat3, phosphorylation of Stat5 and Stat3 were assayed by Western blot analysis. Phosphorylation of Stat5 and Stat3 was further measured by cellular immunofluorescence. The expression plasmid of pcDNA3.1\u2010Stat3 and pcDNA3.1\u2010Stat5b was constructed, respectively. The concentration of interleukin\u20102 (IL\u20102) in the culture supernatants was detected by enzyme\u2010linked immunosorbent assay. ESA inhibited the level of JAK3, phosphorylation of Stat5 and Stat3, and Foxp3 in EL4 cells. The suppressive effects of ESA on Foxp3 were attenuated by forced expression of Stat5 and Stat3. In addition, ESA suppressed IL\u20102R\u03b3 in EL4 cells, while IL\u20102R\u03b3 agonist could markedly reverse the diminished Foxp3 caused by ESA. Furthermore, ESA directly influenced the expression of IL\u20102R\u03b3, rather than the availability of IL\u20102 indirectly. ESA suppressed the level of Foxp3 via inhibiting IL\u20102R\u03b3\/JAK3\/Stats signaling pathway in EL4 cells.<\/jats:p>","DOI":"10.1002\/jcb.27358","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T15:25:50Z","timestamp":1534951550000},"page":"10176-10185","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Excreted\u2010secreted antigens of <i>Toxoplasma gondii<\/i> inhibit Foxp3 via IL\u20102R\u03b3\/JAK3\/Stats pathway"],"prefix":"10.1002","volume":"119","author":[{"given":"Jinling","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pathogen Biology School of Medicine, Nantong University Nantong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiqun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology School of Medicine, Nantong University Nantong China"},{"name":"Department of Central 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