{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T15:43:09Z","timestamp":1784907789402,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T00:00:00Z","timestamp":1535760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81201671"],"award-info":[{"award-number":["81201671"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to find out the underlying mechanism of forskolin (Fsk) and 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX) on glioma stem cells (GSCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of cAMP\u2010related protein CREB and pCREB as well as apoptosis\u2010related proteins were detected through Western blot analysis. The level of proliferation and growth rate of human GSCs was measured through thiazolyl blue tetrazolium bromide assay and stem cells forming sphere assay. The apoptosis\u2010related gene expression was measured through reverse transcription\u2010polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>cAMP signaling pathway was activated in GSCs with Fsk\u2010IBMX administration. Fsk\u2010IBMX could inhibit the proliferation as well as invasion and promote the apoptosis of U87 cells. Besides, U0126 could inhibit MAPK signaling pathway to increase the sensitivity of GSCs to cAMP signaling pathway. As a result, Fsk\u2010IBMX combined with U0126 had more negative effect on GSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The relationship of cAMP and MAPK signaling pathway in GSCs may provide a potential therapeutic strategy in glioma.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27364","type":"journal-article","created":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T10:24:42Z","timestamp":1535797482000},"page":"321-331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Fsk and IBMX inhibit proliferation and proapoptotic of glioma stem cells via activation of cAMP signaling pathway"],"prefix":"10.1002","volume":"120","author":[{"given":"Peng","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Pathophysiology Jilin Medical University Jilin 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