{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:06:30Z","timestamp":1791925590846,"version":"4.3.4"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,8,26]],"date-time":"2018-08-26T00:00:00Z","timestamp":1535241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006096","name":"Shiraz Transplant Research Center, Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["#90\u201010253"],"award-info":[{"award-number":["#90\u201010253"]}],"id":[{"id":"10.13039\/501100006096","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Poor survival of stem cells in the harsh microenvironment at the site of stroke, especially during acute phase of injury, remains a serious obstacle to achieve the desired prognosis. We hypothesized that combined treatment of neural stem cells (NSCs) with small molecules would precondition them to become robust and survive better as compared with the native nonpreconditioned cells. Mouse ganglionic NSCs were isolated, cultured, and characterized. The cells were preconditioned by treatment with sodium butyrate (NaB) and nicorandil (Nico) and transplanted in an experimentally induced stroke model. Sham\u2010operated animals without treatment or animals with experimental stroke treated with basal medium, native NSCs, NSCs preconditioned with NaB or Nico alone were used as controls. The tissue samples and cells with different treatments were used to measure brain\u2010tissue\u2013derived neurotrophic factor (BDNF) level and the activity of phosphatidylinositol\u20103 kinase (PI3K), apurinic\/apyrimidinic endonuclease 1 (APE1), and nuclear factor\u2010\u03baB (NF\u2010\u03baB) p50 both in vitro and in vivo, respectively. Additionally, survival of the cells and recovery indices for stroke were studied. The combined treatment with NaB\u2009+\u2009Nico resulted in increased BDNF level and higher PI3K, APE1, and the downstream NF\u2010\u03baB activation, which were blocked by pretreatment with their respective inhibitors. Donor cell survival increased postengraftment as assessed by 5\u2010bromo\u20102\u2032\u2010deoxyuridine\u00a0immunostaining and reduced Terminal deoxynucleotide transferase dUTP Nick End Labeling positivity at the site of engraftment. There was reduction in proinflammatory cytokines and infiltration of both GFAP\n<jats:sup>+<\/jats:sup> and CD68\n<jats:sup>+<\/jats:sup> at the injury site. There was reduction in the infarct size and neurological function was preserved in the preconditioned cell treatment group. Our preconditioning approach with small molecules effectively improved the survival as well as functionality of NSCs.<\/jats:p>","DOI":"10.1002\/jcb.27372","type":"journal-article","created":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T04:35:19Z","timestamp":1535430919000},"page":"10301-10313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Preconditioned neurons with NaB and nicorandil, a favorable source for stroke cell therapy"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3163-6856","authenticated-orcid":false,"given":"Seyed Mojtaba","family":"Hosseini","sequence":"first","affiliation":[{"name":"Student Research Committee Shiraz University of Medical Sciences Shiraz Iran"},{"name":"Medical Faculty Cell and\u00a0Molecular Medicine Student Research Group, Shiraz University of Medical Sciences Shiraz Iran"},{"name":"Stem Cell Laboratory, Department of Anatomy Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyyed Mohyeddin","family":"Ziaee","sequence":"additional","affiliation":[{"name":"Medical Faculty Cell and\u00a0Molecular Medicine Student Research Group, Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khawaja Husnanin","family":"Haider","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences SRU  Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliashghar","family":"Karimi","sequence":"additional","affiliation":[{"name":"Noncommunicable Diseases Research Center Fasa University of Medical Sciences  Fasa Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Tabeshmehr","sequence":"additional","affiliation":[{"name":"Medical Faculty Cell and\u00a0Molecular Medicine Student Research Group, Shiraz University of Medical Sciences 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