{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:24:04Z","timestamp":1787495044563,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T00:00:00Z","timestamp":1535760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences","award":["75891"],"award-info":[{"award-number":["75891"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The outcome of in vitro maturation (IVM) in the patients with polycystic ovary syndrome (PCOS) is poor. Abnormal intraovarian paracrine interplay alters microenvironment for oocyte development through folliculogenesis and decreases developmental competence of oocytes in\u00a0patients with PCOS. Mesenchymal stromal cells (MSCs) secrete a variety of cytokines and growth factors that could promote oocyte maturation\u00a0in vitro. Thus, in the current study we aimed to evaluate the effect of human bone marrow MSC\u2013conditioned media (hBM\u2010MSC\u2010CM), as a supplement, to enrich IVM medium for PCOS germinal vesicles (GVs). For this purpose, oocytes at GV and metaphase II (MII) stages were harvested from PCOS mice. The GVs were randomly divided into four groups and incubated for 24\u2009hours in an IVM medium (TCM199, as the control group) or TCM199 supplemented by 25%, 50%, and 75% of hBM\u2010MSC\u2010CM (PCOS\u2010CM25, PCOS\u2010CM50, and PCOS\u2010CM75 groups, respectively) so as to evaluate which dose(s) could enhance maturation rate of the GVs and their subsequent in vitro fertilization (IVF) outcome. Furthermore, MII oocytes and their subsequent IVF outcome were considered as the in vivo matured (PCOS\u2010IVO) group. The data showed that supplementation of IVM medium with 50% hBM\u2010MSC\u2010CM significantly increased cytoplasmic and nuclear maturation of the GVs (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001), and also fertilization and two\u2010cell rate (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001) and blastocyst formation (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.01) of in vitro matured oocytes from mice with PCOS. Overall, higher oocyte maturation and fertilization outcome in PCOS\u2010CM50 group proposed that enrichment of IVM medium with hBM\u2010MSC\u2010CM could be considered as a promising approach to improve IVM of PCOS oocytes.\n                  <\/jats:p>","DOI":"10.1002\/jcb.27380","type":"journal-article","created":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T06:59:05Z","timestamp":1535785145000},"page":"10365-10375","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell\u2013conditioned media"],"prefix":"10.1002","volume":"119","author":[{"given":"Hamideh","family":"Jafarzadeh","sequence":"first","affiliation":[{"name":"Department of Biology and Anatomical Sciences, School of Medicine Shahid Beheshti University of Medical Sciences  Tehran 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