{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:17:16Z","timestamp":1740104236355,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The current study aimed to explore the role and the molecular mechanism of Shen\u2019ge powder in cardiomyocyte hypertrophy in chronic heart failure (CHF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sprague\u2010Dawley rats were selected for the study and divided randomly into four groups: control, model, Shen\u2019ge powder, and fasudil group. An inverted microscope was used to determine the diameter of cardiomyocytes in each group. The survival and apoptotic rate of cardiomyocytes in each group was determined by the tetrazolium dye MTT 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide (MTT) assay and flow cytometry, respectively. The messenger RNA levels and protein expression of RhoA, Rho\u2010associated coiled\u2010coil forming protein kinase (ROCK), myosin light\u2010chain phosphatase (MLCP), and myosin light\u2010chain kinase (MLCK) were determined by quantitative reverse transcription\u2010polymerase chain reaction and Western blot analysis, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CHF increased the diameter and apoptotic rate of cardiomyocytes and decreased the survival rate of cardiomyocytes. The levels of RhoA, ROCK, and MLCK were increased significantly in CHF model rats, and the level of MLCP was decreased. After treating with Shen\u2019ge powder, the expression of RhoA, ROCK, and MLCK decreased dramatically and the expression of MLCP increased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Shen\u2019ge powder could reduce cardiomyocyte hypertrophy in CHF by regulating the Rho\/ROCK signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27386","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T10:02:21Z","timestamp":1543226541000},"page":"3038-3045","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Shen\u2019ge powder decreases the cardiomyocyte hypertrophy in chronic heart failure by activating the Rho protein\/Rho\u2010associated coiledcoil forming protein kinase signaling pathway"],"prefix":"10.1002","volume":"120","author":[{"given":"Xinpeng","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine Shengli Oilfield Central Hospital Dongying Shandong PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Cardiology Shengli Oilfield Central Hospital Dongying Shandong PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1792-2402","authenticated-orcid":false,"given":"Xiaozheng","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Cardiology Shengli Oilfield Central Hospital Dongying Shandong PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,26]]},"reference":[{"key":"e_1_2_10_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2010.04.004"},{"key":"e_1_2_10_1_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/11592090-000000000-00000"},{"key":"e_1_2_10_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14282-1"},{"key":"e_1_2_10_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurjhf\/hfs105"},{"key":"e_1_2_10_1_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.2005.068270"},{"key":"e_1_2_10_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2007.04.006"},{"key":"e_1_2_10_1_8_1","first-page":"119","article-title":"Rho\/ROCK and MAPK signaling pathways are involved in glioblastoma cell migration and proliferation","volume":"29","author":"Zohrabian VM","year":"2009","journal-title":"Anticancer Res"},{"key":"e_1_2_10_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2006.01.059"},{"key":"e_1_2_10_1_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.101.5.558"},{"key":"e_1_2_10_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-5151(03)00057-4"},{"volume-title":"Congestive heart failure","year":"2007","author":"Hosenpud JD","key":"e_1_2_10_1_12_1"},{"key":"e_1_2_10_1_13_1","unstructured":"American Heart Association. 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