{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:47:13Z","timestamp":1791499633628,"version":"4.3.4"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,26]],"date-time":"2018-08-26T00:00:00Z","timestamp":1535241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ege University Scientific Research Project","award":["16-TIP-076"],"award-info":[{"award-number":["16-TIP-076"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of Wnt pathway in head and neck cancer could not be elucidated, even though the aberrant Wnt signaling plays a key role in the development of many types of cancer. The inhibitor of \u03b2\u2010catenin responsive transcription (ICRT\u20103) blocks the Wnt signaling pathway by binding to\u00a0\u03b2\u2010catenin, which is a coactivator of the Wnt signaling pathway and\u00a0a promising agent for inhibiting aberrant signaling. In our study, we aimed to evaluate the effect of ICRT\u20103 on the cytotoxicity, apoptosis, cell cycle progression, migration, and gene expressions in head and neck cancer stem cell (HNCSC) and hypopharynx cancer. The effect of this compound on cytotoxicity and cell viability in FaDu and HNCSC line was assessed by using the water\u2010soluble tetrazolium salt\u20101 method. The effect of ICRT\u20103 on apoptosis was detected by using Annexin V and caspase\u20103, caspase\u20109 kit, on cell cycle progression by cycle test plus DNA reagent kit, on gene expression by dual luciferase reporter assay, and on migration activity by wound healing assay in both\u00a0cell lines. ICRT\u20103 was determined to have cytotoxic and apoptotic effect in both\u00a0cell lines. In addition, it was also found that the administration of ICRT\u20103 caused cell cycle arrest and significant decrease in gene expression level and migration ability of the cells.<\/jats:p>","DOI":"10.1002\/jcb.27393","type":"journal-article","created":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T04:35:58Z","timestamp":1535430958000},"page":"380-395","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The effect of ICRT\u20103 on Wnt signaling pathway in head and neck cancer"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1210-7660","authenticated-orcid":false,"given":"Fatma","family":"Sogutlu","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6797-7655","authenticated-orcid":false,"given":"Cagla","family":"Kayabasi","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0659-1097","authenticated-orcid":false,"given":"Besra","family":"Ozmen Yelken","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4123-4175","authenticated-orcid":false,"given":"Aycan","family":"Asik","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roya","family":"Gasimli","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0632-3719","authenticated-orcid":false,"given":"Fatma","family":"Dogan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0535-150X","authenticated-orcid":false,"given":"Sunde","family":"Yilmaz S\u00fcsl\u00fcer","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2748-3124","authenticated-orcid":false,"given":"Cigir","family":"Biray Avc\u0131","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6593-3237","authenticated-orcid":false,"given":"Cumhur","family":"Gunduz","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biology Ege University Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,26]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coms.2014.01.001"},{"key":"e_1_2_8_1_3_1","volume-title":"Essentials Of Head And Neck Cancer","author":"Dwivedi RC","year":"2011"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/J.1525\u20101497.1997.00012.X"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912\u2010005\u20100041\u2010x"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers3010415"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.8256"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405\u2010009\u20101166\u20106"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.31.8311"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.1.42"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2015.07.043"},{"key":"e_1_2_8_1_12_1","unstructured":"IkpeazuCV Treatment of Locally Recurrent and Metastatic Squamous Cell Carcinoma of Head and Neck."},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.5772\/61151"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511423393"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2015.35"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2015.61"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442\u20102042.2007.01866.x"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2015.12.004"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00244"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.15.159"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2012.02.004"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1017496108"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ORALONCOLOGY.2013.03.102"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064088"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.244772.114"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092\u20108674(02)01014\u20100"},{"key":"e_1_2_8_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2012.12.020"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.146977"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010011\u20100879\u20101"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476\u20104598\u201013\u2010209"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12033"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E02\u201002\u20100092"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023000"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023429"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.48"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3630253"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023000"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102407\u201010\u2010437"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062116"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715761003667554"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.262"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.573519"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1924910"},{"key":"e_1_2_8_1_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2342"},{"key":"e_1_2_8_1_45_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986708783497328"},{"key":"e_1_2_8_1_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/23723556.2015.1012976"},{"key":"e_1_2_8_1_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12820"},{"key":"e_1_2_8_1_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms15022157"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.27393","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27393","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.27393","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T16:26:22Z","timestamp":1732811182000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.27393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,26]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1002\/jcb.27393"],"URL":"https:\/\/doi.org\/10.1002\/jcb.27393","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,26]]},"assertion":[{"value":"2018-03-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}