{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T03:32:05Z","timestamp":1768707125415,"version":"3.49.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,23]],"date-time":"2018-09-23T00:00:00Z","timestamp":1537660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As a common disease, the incidence of atherosclerosis (AS) in the world is high. Therefore, we aimed to evaluate the involvement of hydrogen sulfide (H\n<jats:sub>2<\/jats:sub>S)\/cystathionine \u03b3\u2010lyase (CSE) in the pathogenesis of AS as well as their possible signaling pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Enzyme\u2010linked immunosorbent assay, real\u2010time polymerase chain reaction, and Western blot analysis were used to detect the effect of CSE on the expression of inflammatory cytokines, ie, H\n<jats:sub>2<\/jats:sub>S, thioredoxin\u2010interacting protein (TXNIP), NLRP3, apoptosis\u2010associated speck\u2010like protein (ASC), caspase\u20101, and interleukin (IL)\u20101\u03b2. In addition, immunohistochemistry and Western blot analysis were performed to detect the levels of TXNIP, NLRP3, ASC, caspase\u20101, IL\u20101\u03b2, and IL\u201018 among different groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Knockdown of CSE by the transfection of CSE small interfering RNA upregulated the levels of two inflammatory cytokines, ie, IL\u20101\u03b2 and IL\u201018. In addition, the downregulation of CSE promoted the expression of TXNIP, NLRP3, ASC, caspase\u20101, and IL\u20101\u03b2 in THP\u20101 cells. Meanwhile, treating the cells with sodium hydrosulfide (NaHS) inhibited the productions of IL\u20101\u03b2 and IL\u201018. Furthermore, upregulation of H\n<jats:sub>2<\/jats:sub>S synthesis by treating the cells with NaHS also reduced the protein levels of TXNIP, NLRP3, ASC, caspase\u20101, and IL\u20101\u03b2. Finally, the protein levels of TXNIP and NLRP3 in the AS group were much higher than those in the AS\u2009+\u2009H\n<jats:sub>2<\/jats:sub>S group, which in turn was higher than the sham group. In addition, the AS group displayed the highest protein levels of TXNIP, NLRP3, ASC, caspase\u20101, IL\u20101\u03b2, and IL\u201018, while the levels of these proteins in the AS\u2009+\u2009H\n<jats:sub>2<\/jats:sub>S group were higher than those in the sham group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In summary, the present finding suggested a possible linkage between H\n<jats:sub>2<\/jats:sub>S metabolism and AS through the H\n<jats:sub>2<\/jats:sub>S\/CSE\u2010TXNIP\u2010NLRP3\u2010IL\u201018\/IL\u20101\u03b2\u2010nitric oxide (NO) signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27404","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T03:14:09Z","timestamp":1537758849000},"page":"481-492","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation on the effect of hydrogen sulfide on the NLRP3 signaling pathway and its 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