{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T05:40:32Z","timestamp":1778910032978,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T00:00:00Z","timestamp":1534723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Long noncoding RNAs (lncRNAs) have been reported to be involved in several neurological pathogenesis conditions including cerebral ischemia. In the current study, the functions of lncRNA EFNA3 on hypoxia\u2010injured rat adrenal pheochromocytoma (PC\u201012) cells and the underlying molecular mechanism were studied. The expression of lncRNA EFNA3 was silenced by short hairpin RNA transfection, after which the cells were subjected with hypoxia. Cell viability, migration, invasion, and apoptosis were, respectively, determined by trypan blue staining, Transwell assay, annexin V\u2010fluorescein isothiocyanate (FITC)\/propidium iodide (PI) double\u2010staining, and Western blot analysis. The cross regulation between lncRNA EFNA3 and miR\u2010101a, as well as between miR\u2010101a and Rho associated coiled\u2010coil containing protein kinase 2 (ROCK2) were detected by performing quantitative real\u2010time polymerase chain reaction, RNA pull\u2010down, RNA immunoprecipitation, luciferase activity assay, and Western blot analysis. Studies showed that lncRNA EFNA3 was highly expressed in response to hypoxia. Deletion of lncRNA EFNA3 significantly aggravated hypoxia\u2010induced injury in PC\u201012 cells, as the impairment of cell viability, migration, and invasion, and the inducement of apoptosis. LncRNA EFNA3 worked as a sponging molecule for miR\u2010101a and miR\u2010101a suppression\u2010protected PC\u201012 cells against hypoxia\u2010induced injury even when lncRNA EFNA3 was silenced. ROCK2 was a target gene of miR\u2010101a. ROCK2 overexpression exhibited neuroprotective activities. Besides, ROCK2 overexpression activated Wnt\/\u03b2\u2010catenin pathway whereas it deactivated JAK\/STAT pathway upon hypoxia. Our study suggests that deletion of lncRNA EFNA3 aggravates hypoxia\u2010induced injury in PC\u201012 cells by upregulating miR\u2010101a, which further targets ROCK2.<\/jats:p>","DOI":"10.1002\/jcb.27444","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T13:30:17Z","timestamp":1534771817000},"page":"836-847","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Retracted<\/i>: Deletion of long noncoding RNA EFNA3 aggravates hypoxia\u2010induced injury in PC\u201012 cells by upregulation of miR\u2010101a"],"prefix":"10.1002","volume":"120","author":[{"given":"Weijun","family":"Gong","sequence":"first","affiliation":[{"name":"Department of Neurological Rehabilitation Beijing Rehabilitation Hospital, Capital Medical University Beijing 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