{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:31Z","timestamp":1786977931304,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T00:00:00Z","timestamp":1536624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Razi University of Medical Sciences"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and objective<\/jats:title>\n                    <jats:p>Mitochondria play a crucial role in energy metabolism for the survival and motility of sperm during fertilization. The aim of this study was to determine the association of large\u2010scale mitochondrial DNA deletions with abnormal sperm motility and morphology in asthenoteratozoospermic patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>In this case\u2010control study, 41 semen samples were collected from 18 normozoospermic healthy men and 23 asthenoteratozoospermic patients, according to the WHO guidelines. The swim\u2010up technique was used for separation of spermatozoa on the basis of their motility. Long\u2010range polymerase chain reaction (PCR) was used for screening of mitochondrial DNA (mtDNA) large\u2010scale deletions, and primer shift PCR was used for confirmation of deletions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean sperm motility, normal morphology, and progressive motility in asthenoteratozoospermic patients were significantly lower than in the normozoospermic group (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001). There was a positive significant correlation between motility and normal sperm morphology (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001,\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.741). The results of long\u2010range PCR revealed the existence of 4866\u2010bp deletion along with the two common 4977\u2010bp and 7436\u2010bp deleted mtDNA in both groups. However, the frequency of multiple mtDNA deletions in the asthenoteratozoospermic group (15\/23, 65.22%) was significantly higher than that in the normozoospermic group (7\/18, 38.89%). Direct sequencing of the 534\u2010bp PCR product revealed that it was amplified from the mtDNA with a 4866\u2010bp deletion flanked by a seven\u2010nucleotide direct repeat (5\u2032\u2010ACCCCCT\u20103\u2032).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings suggested that these large\u2010scale deletions of mtDNA may be genetic risk factors for poor sperm quality in asthenoteratozoospermia\u2010induced male infertility. Thus, it is necessary to understand the mechanisms behind the generation of these deletions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.27492","type":"journal-article","created":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T02:51:45Z","timestamp":1536720705000},"page":"1958-1968","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Comparison of large\u2010scale deletions of the sperm mitochondrial DNA in normozoospermic and asthenoteratozoospermic men"],"prefix":"10.1002","volume":"120","author":[{"given":"Maryam","family":"Gholinezhad","sequence":"first","affiliation":[{"name":"Department of Biology Faculty of Basic Sciences, Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousefreza","family":"Yousefnia\u2010pasha","sequence":"additional","affiliation":[{"name":"Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences Babol Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abasalt","family":"Hosseinzadeh Colagar","sequence":"additional","affiliation":[{"name":"Departement of Molecular and Cell Biology Faculty of Basic Sciences, University of Mazandaran Babolsar Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8204-5872","authenticated-orcid":false,"given":"Milad","family":"Mohammadoo\u2010Khorasani","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Faculty of Medical Sciences,Tarbiat Modares University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Bidmeshkipour","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Basic Sciences, Razi University Kermanshah 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