{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T23:16:11Z","timestamp":1767136571390,"version":"build-2238731810"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,18]],"date-time":"2018-11-18T00:00:00Z","timestamp":1542499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Optic nerve injury is one of the most common and serious complications in traumatic brain injury (TBI). Alleviating degree of optic nerve injury is important to cure of TBI. This study explored the role of long noncoding RNA (lncRNA) GAS5 in mice retinal ganglion cells (RGCs) suffered to H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      injury.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Primary RGC (PRGCs) were treated with H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      to simulate an in vitro oxidation stress model. LncRNA GAS5 and miR\u2010124 expressions were knocked down by cell transfection with short\u2010hairpin RNA against GAS5 and miR\u2010124 inhibitor, and the transfection efficiency was determined by qRT\u2010PCR. Cell viability, apoptotic cell rate, and production of reactive oxygen species (ROS) was analyzed by CCK\u20108 assay, PI\/FITC\u2010Annexin V method, and DCFH\u2010DA fluorometric assay. Cell apoptosis\u2010associated proteins as well as activations of JAK\/STAT3 signaling and JNK signaling were analyzed by Western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      treatment\u2010induced cell injury was inhibited by lncRNA GAS5 silence. Specifically, knockdown of GAS5 improved viability of primary PRGCs, inhibited apoptosis, decreased ROS expression, increased antiapoptosis proteins\u2019 expressions, and decreased proapoptosis proteins\u2019 expressions. It was also found that miR\u2010124 inhibitor treatment impaired the cell protective effect of GAS5 silence, indicating low level of GAS5 protected PRGCs via upregulating miR\u2010124. GAS5 silence might exert cytoprotection effect via activating JAK\/STAT3 signaling pathway and inhibiting activation of JNK signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Knocking down lncRNA GAS5 alleviated H\n                      <jats:sub>2<\/jats:sub>\n                      O\n                      <jats:sub>2<\/jats:sub>\n                      \u2010induced injury in PRGCs via upregulation of miR\u2010124, which might dependent on activation of JAK\/STAT3 signaling pathway and inhibition of JNK signaling pathway.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.27560","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:50:02Z","timestamp":1542588602000},"page":"2313-2322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>Retracted<\/i>\n                    : Knockdown of long noncoding RNA GAS5 attenuates H\n                    <sub>2<\/sub>\n                    O\n                    <sub>2<\/sub>\n                    \u2010induced damage in retinal ganglion cells through upregulating miR\u2010124: Potential role in traumatic brain injury"],"prefix":"10.1002","volume":"120","author":[{"given":"Xinglu","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Jining No. 1 People's Hospital Jining Shandong 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