{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T14:41:47Z","timestamp":1754145707555,"version":"3.41.2"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T00:00:00Z","timestamp":1536624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W81XWH\u201009\u20101\u20100674","W81XWH\u201015\u20101\u20100079"],"award-info":[{"award-number":["W81XWH\u201009\u20101\u20100674","W81XWH\u201015\u20101\u20100079"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30EY14801"],"award-info":[{"award-number":["P30EY14801"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue response to metallic contact and injury remains poorly understood at the level of proteins and lipid changes. We used corneas as a model system (bovine, porcine [<jats:italic>n<\/jats:italic>\u2009=\u2009300 each], and human corneas [<jats:italic>n<\/jats:italic>\u2009=\u20096]) to characterize proteomic and lipidomic reactions to metallic exposure (impaction with iron). We also made a limited investigation into protein extractability and profile changes due to copper, and lead exposure as well. We identified selected proteins after trypsin digestion using an LCQ Deca XP and lipids using a TSQ Quantum Access Max mass spectrometer, respectively. Our findings indicate that iron impaction to corneal tissue results in cleavage of 1\u2010phosphatidylinositol\u20104,5\u2010bisphosphate phosphodiesterase beta\u20102 variant (PLCB2; 134\u2009kDa) into a 36\u2009kDa species and presence of the epithelial layer is necessary for this cleavage. Penetration of the cornea with other metals (copper and lead) resulted in lower protein extractability from corneal tissue compared with controls but not distinct changes in PLCB2. The changes in protein profiles were unique to the type of metal used for impaction. The depth of injury negatively affected protein extractability and profile compared with controls. These changes were also dependent on several other factors in a complex manner. Iron impaction of corneal tissue for 24\u2009hours results in cleavage of PLCB2 commensurate with significant changes in phosphatidylinositols but not phosphatidylcholines or other phospholipids.<\/jats:p>","DOI":"10.1002\/jcb.27563","type":"journal-article","created":{"date-parts":[[2018,9,12]],"date-time":"2018-09-12T02:24:25Z","timestamp":1536719065000},"page":"2347-2361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tissue protein and lipid alterations in response to metallic impaction"],"prefix":"10.1002","volume":"120","author":[{"given":"Shari A.","family":"Seidman","sequence":"first","affiliation":[{"name":"Bascom Palmer Eye Institute, McKnight Research Center, University of Miami Miami Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha A.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Bascom Palmer Eye Institute, McKnight Research Center, University of Miami Miami Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulises","family":"Arbelo","sequence":"additional","affiliation":[{"name":"Bascom Palmer Eye Institute, McKnight Research Center, University of Miami Miami Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katyayini","family":"Aribindi","sequence":"additional","affiliation":[{"name":"Bascom Palmer Eye Institute, McKnight Research Center, University of Miami Miami Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3759-647X","authenticated-orcid":false,"given":"Sanjoy K","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Bascom Palmer Eye Institute, McKnight Research Center, University of Miami Miami 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