{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T17:16:16Z","timestamp":1767978976373,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T00:00:00Z","timestamp":1540512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Egyptian Science and Technology Development Fund, RSTDG; ID: 22935."}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim and Background<\/jats:title><jats:p>Malignant pleural mesothelioma (MPM) is a lethal cancer mainly caused by chronic exposure of asbestos. In this pilot study, we aimed to assess the expression of serum RNA\u2010based biomarker panel exploring their clinical utility as diagnostic and prognostic biomarkers for MPM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We have selected an MPM\u2010specific RNA\u2010based biomarker panel through bioinformatics analysis based on the integration of DNA damage regulated autophagy modulator 1 (<jats:italic>DRAM1<\/jats:italic>) and arylsulfatase A (\n<jats:italic>ARSA<\/jats:italic>) gene expression with their epigenetic regulators microRNA (\n<jats:italic>miR\u20102053<\/jats:italic>) and long noncoding RNA (\n<jats:italic>lncRNA\u2010RP1\u201086D1.3<\/jats:italic>). Then, quantitative real\u2010time polymerase chain reaction (qPCR) validation in sera of 60 MPM patients, 20 chronic asbestos exposure patients, and 20 healthy volunteers was done. Lastly, the prognostic power of the selected panel was assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of serum <jats:italic>DRAM1<\/jats:italic> messenger RNA (mRNA), \n<jats:italic>ARSA<\/jats:italic> mRNA, \n<jats:italic>hsa\u2010miR\u20102053<\/jats:italic> and \n<jats:italic>lncRNA\u2010RP1\u201086D1.3<\/jats:italic> were positive in 78.3%, 90%, 85%, and 83.3% of MPM patients, respectively. The RNA\u2010based biomarker panel was able to discriminate between MPM patients and controls with high accuracy and their combined sensitivity reached 100% for the diagnosis of MPM. Kaplan\u2010Meier analysis showed that \n<jats:italic>hsa\u2010miR\u20102053<\/jats:italic> is an independent prognostic factor of MPM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our preliminary data revealed that the chosen RNAs play an important role in driving MPM development and progression.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27586","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T08:40:09Z","timestamp":1540543209000},"page":"3203-3211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Clinical significance of serum <i>DRAM1<\/i> mRNA, <i>ARSA<\/i> mRNA, <i>hsa\u2010miR\u20102053<\/i> and <i>lncRNA\u2010RP1\u201086D1.3<\/i> axis expression in malignant pleural mesothelioma"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6852-3954","authenticated-orcid":false,"given":"Marwa","family":"Matboli","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology Faculty of Medicine, Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayman E.","family":"Shafei","sequence":"additional","affiliation":[{"name":"Department of Biomedical Research Armed Forces College of Medicine Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud A.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Biomedical Research Armed Forces College of Medicine Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed I.","family":"Gaber","sequence":"additional","affiliation":[{"name":"Undergraduate Student, Armed Forces College of Medicine Cairo 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