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Various types of mechanical stimuli promote osteogenesis and increase bone formation elicit increases in intracellular\u2010free calcium concentration in osteoblasts. However, whether microgravity, a condition of mechanical unloading, exerts an influence on intracellular\u2010free calcium concentration in osteoblasts or what mechanisms may underlie such an effect are unclear. Herein, we show that simulated microgravity reduces intracellular\u2010free calcium concentration in primary mouse osteoblasts. In addition, simulated microgravity substantially suppresses the activities of L\u2010type voltage\u2010sensitive calcium channels, which selectively allow calcium to cross the plasma membrane from the extracellular space. Moreover, the functional expression of ryanodine receptors and inositol 1,4,5\u2010trisphosphate receptors, which mediate the release of calcium from intracellular storage, decreased under simulated microgravity conditions. These results suggest that simulated microgravity substantially reduces intracellular\u2010free calcium concentration through inhibition of calcium channels in primary mouse osteoblasts. Our study may provide a novel mechanism for microgravity\u2010induced detrimental effects in osteoblasts, offering a new avenue to further investigate bone loss induced by mechanical unloading.<\/jats:p>","DOI":"10.1002\/jcb.27685","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T14:00:53Z","timestamp":1538056853000},"page":"4009-4020","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Simulated microgravity reduces intracellular\u2010free calcium concentration by inhibiting calcium channels in primary mouse osteoblasts"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6558-2174","authenticated-orcid":false,"given":"Zhongyang","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Orthopedics Jinling Hospital, Medical School of Nanjing University Nanjing China"},{"name":"Department of Orthopedics No. 454 Hospital of PLA, Anhui 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