{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T21:38:17Z","timestamp":1768685897957,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T00:00:00Z","timestamp":1538006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007162","name":"Guangdong Science and Technology Department","doi-asserted-by":"publisher","award":["2014A020212647"],"award-info":[{"award-number":["2014A020212647"]}],"id":[{"id":"10.13039\/501100007162","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acetaminophen (APAP) is a widely used over\u2010the\u2010counter analgesic and antipyretic. It can cause hepatotoxicity. Recent studies demonstrated that hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) exhibits cell protection in several cell types. This study was designed to investigate whether H\n<jats:sub>2<\/jats:sub>S ameliorated APAP\u2010induced acute liver injury and to elucidate its mechanisms. In this study, we analyzed the detailed biological and molecular processes of APAP\u2010induced hepatotoxicity using a bioinformatics analysis, which showed that apoptosis and the c\u2010Jun N\u2010terminal kinase (JNK)\/mitogen\u2010activated protein kinase pathway were confirmed to play critical roles in these processes. We further investigated the protective effects of H\n<jats:sub>2<\/jats:sub>S on APAP\u2010induced hepatotoxicity. In vivo, we observed that the exogenous supplement of H\n<jats:sub>2<\/jats:sub>S ameliorated APAP\u2010induced liver injury. Cystathionine\u2010\u03b2\u2010synthase (CBS) and cystathionine\u2010\u03b3\u2010lyase (CSE) systems were the endogenous pathway of H\n<jats:sub>2<\/jats:sub>S. The expression of CBS\/CSE was decreased in APAP\u2010treated mice, while H\n<jats:sub>2<\/jats:sub>S could significantly restore it. In addition, APAP\u2010induced JNK activation was inhibited by H\n<jats:sub>2<\/jats:sub>S in vivo. In vitro, H\n<jats:sub>2<\/jats:sub>S abolished the active effects of APAP on caspase3, Bax, and Bcl\u20102 expressions as well as JNK phosphorylation in hepatocytes. It was found through flow cytometry that the amount of APAP\u2010induced apoptotic hepatocytes was decreased in the presence of H\n<jats:sub>2<\/jats:sub>S. In conclusion, our results suggested that H\n<jats:sub>2<\/jats:sub>S attenuated APAP\u2010induced apoptosis in hepatocytes through JNK\/MAPK siganaling pathway.<\/jats:p>","DOI":"10.1002\/jcb.27724","type":"journal-article","created":{"date-parts":[[2018,9,27]],"date-time":"2018-09-27T13:18:41Z","timestamp":1538054321000},"page":"4385-4397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Hydrogen sulfide protects against acetaminophen\u2010induced acute liver injury by inhibiting apoptosis via the JNK\/MAPK signaling pathway"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7754-731X","authenticated-orcid":false,"given":"Xiaoyong","family":"Li","sequence":"first","affiliation":[{"name":"Department of General Surgery The Third Affiliated hospital of Guangzhou Medical University, Guangzhou Medical University Guangzhou Guangdong 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