{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T08:44:21Z","timestamp":1782809061717,"version":"3.54.5"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T00:00:00Z","timestamp":1539561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Oridonin is a diterpenoid isolated from <jats:italic>Rabdosia rubescens<\/jats:italic> with potent anticancer activities. Valproic acid (VPA) is a recently emerged antineoplastic histone deacetylase inhibitor. The aim of the present study is to investigate the synergistic role of oridonin and VPA to inhibit the growth and metastasis of human leukemia cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The effect of oridonin and VPA on proliferation was evaluated by the MTT assay. Cell migration and invasion were evaluated by transwell and scratch assays, respectively. In addition, cell apoptosis was examined by flow cytometry. The inhibitive effects of oridonin and VPA in vivo were determined by using xenografted nude mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results demonstrated that oridonin in combination with VPA synergistically inhibited the proliferation of HL\u201060 cells, and induced obvious caspase\u2010dependent apoptosis through activation of the intrinsic apoptosis pathway, which is involved in the downregulation of Bcl\u20102\/Bax ratio. Furthermore, the combination treatment in vivo remarkably reduced the xenograft tumor size and triggered tumor cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggested that the novel combination of oridonin plus VPA exerted synergistic antiproliferative and apoptosis\u2010inducing effects on human myeloid leukemia cells, and may serve as a potentially promising antileukemia strategy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27845","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T15:29:10Z","timestamp":1539617350000},"page":"5620-5627","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Synergistic antitumor activity of oridonin and valproic acid on HL\u201060 leukemia cells"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5433-5402","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"first","affiliation":[{"name":"Department of hematology Hunan Province People's Hospital Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LiEn","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of hematology Hunan Province People's Hospital Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,15]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-013-1788-z"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/shem.2001.20863"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V72.2.567.567"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.4084\/mjhid.2012.012"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-002-0031-1"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000046623"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.3.759"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1521-6926(03)00040-9"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-0209"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X13500134"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-10-610"},{"key":"e_1_2_6_1_13_1","first-page":"1793","article-title":"Oridonin nanosuspension was more effective than free oridonin on G2\/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC\u20101 cell line","volume":"7","author":"Qi X","year":"2012","journal-title":"Int J Nanomedicine"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2012.895"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.859"},{"issue":"1","key":"e_1_2_6_1_16_1","first-page":"139","article-title":"Synergistic antitumor activity of oridonin and arsenic trioxide on hepatocellular carcinoma cells","volume":"40","author":"Chen G","year":"2012","journal-title":"Int J Oncol"},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.15.1210"},{"key":"e_1_2_6_1_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-2726"},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403390"},{"key":"e_1_2_6_1_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003562"},{"key":"e_1_2_6_1_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-04-0277"},{"key":"e_1_2_6_1_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.105.012765"},{"key":"e_1_2_6_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(01)00097-8"},{"key":"e_1_2_6_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403828"},{"key":"e_1_2_6_1_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101287200"},{"key":"e_1_2_6_1_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2009.01147.x"},{"key":"e_1_2_6_1_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-06-032250"},{"key":"e_1_2_6_1_28_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.58.3.10"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.27845","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27845","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.27845","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27845","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T20:46:44Z","timestamp":1715028404000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.27845"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,15]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/jcb.27845"],"URL":"https:\/\/doi.org\/10.1002\/jcb.27845","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,15]]},"assertion":[{"value":"2018-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}