{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T08:43:58Z","timestamp":1768812238394,"version":"3.49.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T00:00:00Z","timestamp":1539561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Atomic Energy Regulatory Board (AERB) Department of Atomic Energy, Government of India, India","award":["AERB\/CSRP\/Proj.No.58\/04\/2014"],"award-info":[{"award-number":["AERB\/CSRP\/Proj.No.58\/04\/2014"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In vitro human lymphocyte culture methodology is well established yet certain confounding factors such as age, medical history as well as individual\u2019s blood type may potentially modulate in vitro proliferation response. These factors have to be carefully evaluated to release reliable test report in routine cytogenetic evaluation for various genetic conditions, radiation biodosimetry, etc. With this objective, the current study was focused on analyzing the proliferation response of lymphocytes drawn from 90 individuals (21\u201029 years) with different blood types. The proliferation response was assessed in the cultured lymphocytes by cell cycle, mitotic index (MI), and nuclear division index (NDI) after stimulation with phytohaemagglutinin (PHA). To investigate the toxic effect on proliferation, MI was calculated in representative samples of each blood type were X\u2010irradiated. The results showed that there was no significant difference among the cell cycle phases of lymphocytes in different blood types (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). Similarly, both MI and NDI of lymphocytes derived from different blood types also did not show significant difference (\n<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). The extensive interindividual variation within and among the blood types is likely responsible for the lack of significant difference in lymphocyte proliferation. Although spontaneous proliferation efficiency of lymphocytes of different blood types after PHA stimulation was grossly similar, the MI observed after radiation exposure showed a significant difference (\n<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) indicating a differential proliferation response among the blood types. Our results suggest that the blood types did not have any impact on PHA\u2010induced proliferation; however, a specific differential lymphocyte proliferation observed after radiation exposure needs to be considered.<\/jats:p>","DOI":"10.1002\/jcb.27858","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T15:26:12Z","timestamp":1539617172000},"page":"5722-5728","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Does proliferation capacity of lymphocytes depend on human blood types?"],"prefix":"10.1002","volume":"120","author":[{"given":"Sribala","family":"Viswanathan","sequence":"first","affiliation":[{"name":"Department of Human Genetics Sri Ramachandra Medical College and Research Institute (Deemed to be University) Porur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karthik","family":"Kanagaraj","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Sri Ramachandra Medical College and Research Institute (Deemed to be University) Porur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Venkateswarlu","family":"Raavi","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Sri Ramachandra Medical College and Research Institute (Deemed to be University) Porur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanmugapriya","family":"Dhanasekaran","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Sri Ramachandra Medical College and Research Institute (Deemed to be University) Porur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinod Kumar","family":"Panicker","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Sri Ramachandra Medical College &amp; Research Institute (Deemed to be University) Porur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishnamoorthy","family":"R","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Sri Ramachandra Medical College &amp; Research Institute (Deemed to be University) Porur India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adayabalam","family":"S. Balajee","sequence":"additional","affiliation":[{"name":"Cytogenetics Biodosimetry Laboratory, REAC\/TS, Oak Ridge Associated Universities, Oak Ridge Institute for Science and Education Oak Ridge Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2376-3348","authenticated-orcid":false,"given":"Venkatachalam","family":"Perumal","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Sri Ramachandra Medical College and Research Institute (Deemed to be University) Porur India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,15]]},"reference":[{"key":"e_1_2_9_1_2_1","first-page":"103","article-title":"A minute chromosome in human chronic granulocytic leukemia","volume":"132","author":"Nowell P","year":"2004","journal-title":"Landmarks in Med Genet"},{"key":"e_1_2_9_1_3_1","first-page":"232","volume-title":"Applications in Preparedness for and Response to Radiation Emergencies","author":"Dosimetry C","year":"2011"},{"key":"e_1_2_9_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(88)90017-6"},{"key":"e_1_2_9_1_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V19.3.349.349"},{"key":"e_1_2_9_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(03)00076-7"},{"key":"e_1_2_9_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-2280(2001)37:1<31::AID-EM1004>3.0.CO;2-P"},{"key":"e_1_2_9_1_8_1","first-page":"235","article-title":"Comparing seven mitogens with PHA\u2010M for improved lymphocyte stimulation in dicentric chromosome analysis for biodosimetry","volume":"168","author":"Beinke C","year":"2016","journal-title":"Radiat Prot Dosim"},{"key":"e_1_2_9_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01959890"},{"key":"e_1_2_9_1_10_1","first-page":"295","article-title":"The human leukocyte test system. 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