{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:57:27Z","timestamp":1787169447765,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T00:00:00Z","timestamp":1541116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Emerging evidence has suggested that microRNAs play a critical role in neuropathic pain development. However, the biological role of miRNAs in regulating neuropathic pain remains barely known. In our present study, we found that miR\u2010124\u20103p was significantly downregulated in rats after chronic sciatic nerve injury (CCI). In addition, it was showed that overexpression of miR\u2010124\u20103p obviously repressed mechanical allodynia and heat hyperalgesia. Meanwhile, it has been reported that neuroinflammation can contribute a lot to neuropathic pain progression. Here, we found that inflammatory cytokine (IL\u20106, IL\u20101\u03b2, and TNF\u2010\u237a) protein expression in rats after CCI greatly increased and miR\u2010124\u20103p mimics depressed inflammation cytokine levels. Consistently, miR\u2010124\u20103p alleviated inflammation production in lipopolysaccharide\u2010incubated spinal microglial cells. Bioinformatics analysis revealed that EZH2 acted as a direct target of miR\u2010124\u20103p, which participated in the miR\u2010124\u20103p\u2010modulated effects on neuropathic pain development and neuroinflammation. We observed that miR\u2010124\u20103p was able to promote neuroinflammation and neuropathic pain through targeting EZH2. The direct correlation between them was validated in our current study using dual\u2010luciferase reporter assays. Subsequently, it was manifested that EZH2 abrogated the inhibitory role of miR\u2010124\u20103p on neuropathic pain progression in CCI rats. Taken these together, our findings highlighted a novel contribution of miR\u2010124\u20103p to neuropathic pain and indicated the possibilities for developing novel therapeutic options for neuropathic pain.<\/jats:p>","DOI":"10.1002\/jcb.27861","type":"journal-article","created":{"date-parts":[[2018,11,3]],"date-time":"2018-11-03T00:07:09Z","timestamp":1541203629000},"page":"5747-5755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["miR\u2010124\u20103p attenuates neuropathic pain induced by chronic sciatic nerve injury in rats via targeting EZH2"],"prefix":"10.1002","volume":"120","author":[{"given":"Yang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology The Affiliated Huaian No.1 People\u2019s Hospital of Nanjing Medical University Huai\u2019an Jiangsu 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