{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T17:25:54Z","timestamp":1772558754541,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T00:00:00Z","timestamp":1539561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Orientia tsutsugamushi<\/jats:italic>, a cause of scrub typhus is emerging as an important pathogen in several parts of the tropics. The control of this infection relies on rapid diagnosis, specific treatment, and prevention through vector control. Development of a vaccine for human use would be very important as a public health measure. Antibody and T\u2010cell response have been found to be important in the protection against scrub typhus. This study was undertaken to predict the peptide vaccine that elicits both B\u2010 and T\u2010cell immunity. The outer\u2010membrane protein, 47\u2010kDa high\u2010temperature requirement A was used as the target protein for the identification of protective antigen(s). Using BepiPred2 program, the potential B\u2010cell epitope PNSSWGRYGLKMGLR with high conservation among \n<jats:italic>O. tsutsugamushi<\/jats:italic> and the maximum surface exposed residues was identified. Using IEDB, NetMHCpan, and NetCTL programs, T\u2010cell epitopes MLNELTPEL and VTNGIISSK were identified. These peptides were found to have promiscuous class\u2010I major histocompatibility complex (MHC) binding affinity to MHC supertypes and high proteasomal cleavage, transporter associated with antigen processing prediction, and antigenicity scores. In the I\u2010TASSER generated model, the C\u2010score was \u22120.69 and the estimated TM\u2010score was 0.63\u2009\u00b1\u20090.14. The location of the epitope in the 3D model was external. Therefore, an antibody to this outer\u2010membrane protein epitope could opsonize the bacterium for clearance by the reticuloendothelial system. The T\u2010cell epitopes would generate T\u2010helper function. The B\u2010cell epitope(s) identified could be evaluated as antigen(s) in immunodiagnostic assays. This cocktail of three peptides would elicit both B\u2010 and T\u2010cell immune response with a suitable adjuvant and serve as a vaccine candidate.<\/jats:p>","DOI":"10.1002\/jcb.27872","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T15:23:05Z","timestamp":1539616985000},"page":"5869-5879","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of B\u2010 and T\u2010cell epitopes on HtrA protein of <i>Orientia tsutsugamushi<\/i>"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9389-5231","authenticated-orcid":false,"given":"Sathish","family":"Sankar","sequence":"first","affiliation":[{"name":"Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre Vellore Tamil Nadu 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