{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T02:05:58Z","timestamp":1793066758923,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T00:00:00Z","timestamp":1540425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the last three decades, many new cell\u2010penetrating peptides (CPPs) were developed that exhibited enhanced cell selectivity. Thus, we aimed to validate the tumor cell selectivity of peptides from this new generation, namely fragments mini\u2010crotamine and mini\u2010maurocalcine. Both of these peptides are derived from venoms. Furthermore, we studied an analog of the classical CPP HIV\u2010TAT(47\u201057) with alternating chirality of Arg residues. To allow covalent coupling of cargoes or fluorophores, a cysteine residue was introduced to the N\u2010terminus of the synthesized peptides. The therapeutic antibody trastuzumab conjugated to different fluorescent dyes was used for internalization studies. Comparison of uptake efficiencies revealed that CPPs of the new generation are in contrast to MPG\u2010peptides, nearly unable to internalize the noncovalently formed complexes with trastuzumab. Interestingly, the fluorescent derivative of the crotamine fragment was mainly observed in a subpopulation of breast cancer cells, whereas it was homogenously distributed in fibrosarcoma, colon cancer, and noncancerous endothelia cells. Thus, the fluorescent crotamine fragment reported herein is a potent theranostic tool for image\u2010guided applications. This peptide can be used to pinpoint the level of heterogeneity present within tumors and aid in the generation of therapeutics that target heterogenic subpopulations.<\/jats:p>","DOI":"10.1002\/jcb.27943","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T02:43:51Z","timestamp":1540521831000},"page":"6528-6541","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["New generation CPPs show distinct selectivity for cancer and noncancer cells"],"prefix":"10.1002","volume":"120","author":[{"given":"Felista L.","family":"Tansi","sequence":"first","affiliation":[{"name":"Department of Experimental Radiology, Institute of Diagnostic and Interventional Radiology Jena University Hospital Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita P.","family":"Filatova","sequence":"additional","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitri O.","family":"Koroev","sequence":"additional","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga M.","family":"Volpina","sequence":"additional","affiliation":[{"name":"Shemyakin\u2010Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffi","family":"Lange","sequence":"additional","affiliation":[{"name":"Jena Bioscience GmbH Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Schumann","sequence":"additional","affiliation":[{"name":"Ernst\u2010Abbe\u2010University of Applied Sciences Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf K.","family":"Teichgr\u00e4ber","sequence":"additional","affiliation":[{"name":"Department of Experimental Radiology, Institute of Diagnostic and Interventional Radiology Jena University Hospital Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1083-5672","authenticated-orcid":false,"given":"Siegmund","family":"Reissmann","sequence":"additional","affiliation":[{"name":"Jena Bioscience GmbH Jena Germany"},{"name":"Centrum of Molecular Biomedicine, Institute of Biochemistry and Biophysics, Friedrich\u2010Schiller\u2010University Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Hilger","sequence":"additional","affiliation":[{"name":"Department of Experimental Radiology, Institute of Diagnostic and Interventional Radiology Jena University Hospital Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,25]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23020295"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2016.11.011"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.2672"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn4057269"},{"issue":"2","key":"e_1_2_7_1_6_1","first-page":"211","article-title":"Cell\u2010penetrating peptides: A promising tool for the cellular uptake of macromolecular drugs","volume":"19","author":"Zhu P","year":"2018","journal-title":"Curr. Prot. Pept. Sci"},{"key":"e_1_2_7_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00427-13"},{"key":"e_1_2_7_1_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph5090991"},{"key":"e_1_2_7_1_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.115.223305"},{"key":"e_1_2_7_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2017.09.006"},{"key":"e_1_2_7_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)34947-0"},{"key":"e_1_2_7_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915999"},{"key":"e_1_2_7_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90263-2"},{"key":"e_1_2_7_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34080-2"},{"key":"e_1_2_7_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25075"},{"key":"e_1_2_7_1_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604876200"},{"key":"e_1_2_7_1_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161211798999429"},{"key":"e_1_2_7_1_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm8009475"},{"key":"e_1_2_7_1_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2012.01377.x"},{"key":"e_1_2_7_1_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc200421z"},{"key":"e_1_2_7_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.22610"},{"key":"e_1_2_7_1_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp2000605"},{"key":"e_1_2_7_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2016.01.013"},{"key":"e_1_2_7_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.21964"},{"key":"e_1_2_7_1_25_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.2011.602064"},{"key":"e_1_2_7_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01239-4"},{"key":"e_1_2_7_1_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph6030320"},{"key":"e_1_2_7_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-008-9782-1"},{"key":"e_1_2_7_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.10.008"},{"key":"e_1_2_7_1_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/10717544.2018.1435747"},{"key":"e_1_2_7_1_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph5111177"},{"key":"e_1_2_7_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.07.022"},{"key":"e_1_2_7_1_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-0118"},{"key":"e_1_2_7_1_34_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-191536"},{"key":"e_1_2_7_1_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/86684"},{"key":"e_1_2_7_1_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.360628"},{"key":"e_1_2_7_1_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161126054"},{"key":"e_1_2_7_1_38_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-1459fje"},{"key":"e_1_2_7_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.725"},{"key":"e_1_2_7_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23313"},{"key":"e_1_2_7_1_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph6020184"},{"key":"e_1_2_7_1_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1356"},{"key":"e_1_2_7_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(94)00159-U"},{"key":"e_1_2_7_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2010.01.022"},{"key":"e_1_2_7_1_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108795108"},{"key":"e_1_2_7_1_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07798.x"},{"key":"e_1_2_7_1_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21218"},{"key":"e_1_2_7_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.04.111"},{"key":"e_1_2_7_1_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp400680d"},{"key":"e_1_2_7_1_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.104919"},{"key":"e_1_2_7_1_51_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/675985"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.27943","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27943","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.27943","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27943","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T20:43:12Z","timestamp":1715028192000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.27943"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,25]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/jcb.27943"],"URL":"https:\/\/doi.org\/10.1002\/jcb.27943","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,25]]},"assertion":[{"value":"2018-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}