{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:03:17Z","timestamp":1790330597472,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,11]],"date-time":"2018-11-11T00:00:00Z","timestamp":1541894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Liver tissue engineering as a therapeutic option for restoring of damaged liver function has a special focus on using native decellularized liver matrix, but there are limitations such as the shortage of liver donor. Therefore, an appropriate alternative scaffold is needed to circumvent the donor shortage. This study was designed to evaluate hepatic differentiation of human induced pluripotent stem cells (hiPSCs) in decellularized Wharton's jelly (WJ) matrix as an alternative for native liver matrix. WJ matrices were treated with a series of detergents for decellularization. Then hiPSCs were seeded into decellularized WJ scaffold (DWJS) for hepatic differentiation by a defined induction protocol. The DNA quantitative assay and histological evaluation showed that cellular and nuclear materials were efficiently removed and the composition of extracellular matrix was maintained. In DWJS, hiPSCs\u2010derived hepatocyte\u2010like cells (hiPSCs\u2010Heps) efficiently entered into the differentiation phase (G1) and gradually took a polygonal shape, a typical shape of hepatocytes. The expression of hepatic\u2010associated genes (<jats:italic>albumin<\/jats:italic>, <jats:italic>TAT<\/jats:italic>, <jats:italic>Cytokeratin19<\/jats:italic>, and <jats:italic>Cyp7A1<\/jats:italic>), albumin and urea secretion in hiPSCs\u2010Heps cultured into DWJS was significantly higher than those cultured in the culture plates (2D). Altogether, our results suggest that DWJS could provide a proper microenvironment that efficiently promotes hepatic differentiation of hiPSCs.<\/jats:p>","DOI":"10.1002\/jcb.27965","type":"journal-article","created":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T06:15:16Z","timestamp":1542003316000},"page":"6683-6697","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Decellularized Wharton's jelly extracellular matrix as a promising scaffold for promoting hepatic differentiation of human induced pluripotent stem cells"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4296-7757","authenticated-orcid":false,"given":"Mousa","family":"Kehtari","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, School of Biology, College of Science, University of Tehran Tehran Iran"},{"name":"Department of Stem Cell Biology Stem Cell Technology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahareh","family":"Beiki","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, School of Biology, College of Science, University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5112-4287","authenticated-orcid":false,"given":"Bahman","family":"Zeynali","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, School of Biology, College of Science, University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh Sadat","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Biology Stem Cell Technology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Soleimanifar","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Kaabi","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Biology Stem Cell Technology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"Soleimani","sequence":"additional","affiliation":[{"name":"Department of Hematology Faculty of Medical Sciences, Tarbiat Modares University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4516-5944","authenticated-orcid":false,"given":"Seyed Ehsan","family":"Enderami","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Biology Stem Cell Technology Research Center Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahboubeh","family":"Kabiri","sequence":"additional","affiliation":[{"name":"Department of Biotechnology College of Science, University of Tehran Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Mahboudi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Pharmacy, Alborz University of Medical Sciences Karaj Iran"},{"name":"Dietary Supplements and Probiotic Center Alborz University of Medical Sciences Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,11]]},"reference":[{"key":"e_1_2_8_1_2_1","unstructured":"American Liver Foundation.2017.The liver lowdown \u2013liver disease: the big picture."},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2012.12.005"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40472-016-0084-y"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.16.070196.001143"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/8\/1\/015007"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1684"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2193"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189345"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2010.0698"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2170"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3154"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476278.2015.1011908"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2015.0313"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2015-0235"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172098"},{"key":"e_1_2_8_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.04.074"},{"key":"e_1_2_8_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4004(98)90104-7"},{"key":"e_1_2_8_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-2115(96)02390-1"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.10.008"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X654939"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.22270"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.3.9.1552"},{"key":"e_1_2_8_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcma.2013.07.007"},{"key":"e_1_2_8_1_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000446211"},{"key":"e_1_2_8_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.05.009"},{"issue":"4","key":"e_1_2_8_1_27_1","first-page":"1","article-title":"Improvement of hepatogenic differentiation of iPS cells on an aligned polyethersulfone compared to random nanofibers","volume":"46","author":"Mahmoodinia Maymand M","year":"2017","journal-title":"Artif Cells Nanomed Biotechnol"},{"key":"e_1_2_8_1_28_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368911X580572"},{"key":"e_1_2_8_1_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI68793"},{"key":"e_1_2_8_1_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3307"},{"key":"e_1_2_8_1_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054058"},{"key":"e_1_2_8_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200700206"},{"key":"e_1_2_8_1_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0292"},{"key":"e_1_2_8_1_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2018.1443466"},{"key":"e_1_2_8_1_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2018.1452753"},{"key":"e_1_2_8_1_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12265-011-9304-0"},{"key":"e_1_2_8_1_37_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0168"},{"key":"e_1_2_8_1_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2012.0334"},{"key":"e_1_2_8_1_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2004.10.1046"},{"key":"e_1_2_8_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24067"},{"key":"e_1_2_8_1_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13079"},{"key":"e_1_2_8_1_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.12088"},{"key":"e_1_2_8_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4004(97)90071-0"},{"key":"e_1_2_8_1_44_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2012-0160"},{"key":"e_1_2_8_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2012.10.004"},{"key":"e_1_2_8_1_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.07.007"},{"key":"e_1_2_8_1_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25721"},{"key":"e_1_2_8_1_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476278.2016.1185584"},{"key":"e_1_2_8_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.01.024"},{"key":"e_1_2_8_1_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24012"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcb.27965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27965","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.27965","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.27965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T20:45:46Z","timestamp":1715028346000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.27965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,11]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/jcb.27965"],"URL":"https:\/\/doi.org\/10.1002\/jcb.27965","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,11]]},"assertion":[{"value":"2018-07-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}