{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:04:33Z","timestamp":1772269473208,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>This study aimed to investigate the potential function of <jats:italic>FER1L4<\/jats:italic> in the progression of hepatocellular carcinoma and uncover its underlying molecular mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the current study, quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was conducted to determine the expression profile of <jats:italic>FER1L4<\/jats:italic> in normal liver tissues and hepatocellular carcinoma tissues of human, as well as hepatocellular carcinoma (HCC) cell lines including HL\u20107702[L\u201002], HepG\u20102, Hep3b, and SMMC\u20107721. Then, HepG\u20102 cells were transfected with pcDNA3.1\u2010<jats:italic>FER1L4<\/jats:italic> (pcDNA3.1\u2010empty as negative control) for gain\u2010of\u2010function analysis, followed with cell functional abnormality tests. Specifically, colony formation analysis and 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyl\u2010tetrazolium bromide experiment were taken advantage to measure the cell proliferation, while cell migration and invasion were evaluated by wound healing assay and transwell experiment respectively. Additionally, cell apoptosis was detected by flow cytometry. Moreover, the effect of <jats:italic>FER1L4<\/jats:italic> on PI3K\/AKT signal pathway activation was investigated through analyzing phosphorylation of related proteins, p\u2010AKT\/AKT and p\u2010PI3K\/PI3K, via Western blot assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Downregulation of <jats:italic>FER1L4<\/jats:italic> in hepatocellular carcinoma tissues and cells was demonstrated by qRT\u2010PCR analysis. Besides, <jats:italic>FER1L4<\/jats:italic> overexpression evidently attenuated the cell proliferation, migration and invasion, but prompted cell apoptosis. Importantly, Western blot assays revealed that PII3K\/AKT signal pathway were involved in mediating the progression regulation role of <jats:italic>FER1L4<\/jats:italic> in HCC cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study suggested that <jats:italic>FER1L4<\/jats:italic> might alleviate progression of hepatocellular carcinoma via blocking PI3K\/AKT pathway, which encourages a better understanding of the pathogenesis of HCC and may provide a novel potential therapeutic target for clinical treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.27980","type":"journal-article","created":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T10:50:30Z","timestamp":1541069430000},"page":"6781-6788","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Upregulation of lncRNA <i>FER1L4<\/i> suppresses the proliferation and migration of the hepatocellular carcinoma via regulating 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