{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:08:46Z","timestamp":1788944926251,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Erectile dysfunction (ED) is a common sexual problem for men and the exploration of its treatment is still in mire demand. We aim to investigate the role of the Toll\u2010like receptor 4 (TLR4)\/myeloid differentiation primary response gene 88 (MyD88) signaling pathway in the pathogenesis of angiotensin II (Ang\u2010II) induced ED.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Male Sprague\u2010Dawlay rats were treated with Ang\u2010II and intracavernous pressure (ICP) was measured to confirm the occurrence of ED. The corpus cavernosum penises of rats were transfected with plasmids to overexpressed MyD88. Inflammatory and vascular parameters including myeloperoxidase (MPO), cyclooxygenase2 (COX2), endothelial nitric oxide synthase (eNOS), malondialdehyde (MDA), superoxide dismutase (SOD), reactive oxygen species (ROS), and cytokines in treated and untreated ED rats were measured. Flow cytometry was used to determine the apoptosis of endothelial cells of corpus cavernosum penises of rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Ang\u2010II\u2010induced ED rats were found to contain upregulated TLR4, MyD88, MPO, and COX2, and downregulated eNOS. MyD88 overexpression deteriorates cavernous structural damage, reduces ICP and ICP\/MAP values and reverses the therapeutic effect of anti\u2010TLR4 antibodies in rats with Ang\u2010II\u2010induced ED. Moreover, overexpression of MyD88 further upregulated MPO and COX2, downregulated eNOS, promoted oxidative stress, inflammation, and cell apoptosis rate via positively regulating the TLR4\/MyD88 signaling pathway, while anti\u2010TLR4 antibodies downregulated MPO and COX2, upregulated eNOS, suppressed oxidative stress, inflammation, and cell apoptosis rate via inactivating the TLR4\/MyD88 signaling pathway in the rat corpus cavernosum penises. Furthermore, MyD88 overexpression promotes oxidative stress and inflammation and reverses the effect of anti\u2010TLR4 antibodies in the penis of ED rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MyD88 overexpression deteriorates Ang\u2010II\u2010induced ED via upregulating MPO and COX2 and downregulating eNOS in the corpus cavernosum rats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.27987","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T11:25:05Z","timestamp":1543404305000},"page":"7133-7146","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["MyD88 overexpression deteriorates Ang\u2010II\u2010induced ED via upregulating MPO and COX2 and downregulating eNOS in the corpus cavernosum of rats"],"prefix":"10.1002","volume":"120","author":[{"given":"Haishan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery China\u2010Japan Union Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery China\u2010Japan Union Hospital of Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linpei","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Nephrology Xuanwu Hospital of Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0485-4981","authenticated-orcid":false,"given":"Hai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology China\u2010Japan Union Hospital of Jilin University Changchun 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