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Clinical conditions such as ischemia and sleep apnea and extreme physiological conditions such as high\u2010altitude sickness are presented with renal hypoxia and are associated with significant proteinuria. Hypoxia is considered as an etiological factor in the progression of acute renal injury. A sustained increase in hypoxia\u2010inducible factor 1\u03b1 (HIF1\u03b1) is a major adaptive stimulus to the hypoxic conditions. Although the temporal association between hypoxia and proteinuria is known, the mechanism by which hypoxia elicits proteinuria remains to be investigated. Furthermore, stabilization of HIF1\u03b1 is being considered as a therapeutic option to treat anemia in patients with chronic kidney disease. Therefore, in this study, we induced stabilization of HIF1\u03b1 in glomerular regions in vivo and in podocytes in vitro upon exposure to cobalt chloride. The elevated HIF1\u03b1 expression is concurrence with diminished expression of nephrin and podocin, podocyte foot\u2010processes effacement, and significant proteinuria. Podocytes exposed to cobalt chloride lost their arborized morphology and cell\u2010cell connections and also displayed cytoskeletal derangements. Elevation in expression of HIF1\u03b1 is in concomitance with loss of nephrin and podocin in patients with diabetic nephropathy and chronic kidney disease. In summary, the current study suggests that HIF1\u03b1 stabilization impairs podocyte function vis\u2010\u00e0\u2010vis glomerular permselectivity.<\/jats:p>","DOI":"10.1002\/jcb.28041","type":"journal-article","created":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T01:08:16Z","timestamp":1541120896000},"page":"7667-7678","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Stabilization of hypoxia\u2010inducible factor 1\u03b1 by cobalt chloride impairs podocyte morphology and slit\u2010diaphragm function"],"prefix":"10.1002","volume":"120","author":[{"given":"Krishnamurthy","family":"Nakuluri","sequence":"first","affiliation":[{"name":"Department of Biochemistry School of Life Sciences, University of Hyderabad Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhanunjay","family":"Mukhi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry School of Life 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