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An earlier report has shown that exogenous DNase 1 offers protection against such severe local tissue necrosis.\n                    <jats:italic>Tricosanthus tricuspidata<\/jats:italic>\n                    is a medicinal plant and the paste prepared from its leaves has been used extensively for the treatment of snakebite\u2010induced tissue necrosis. Most studies including reports from our laboratory focused on plant secondary metabolite as therapeutic molecules against snakebite envenomation. However, the involvement of hydrolytic enzymes including DNase in treating snake venom\u2010induced tissue necrosis has not been addressed. Several folk medicinal plants used against snakebite treatment showed the presence of DNase activity and found to be rich in\n                    <jats:italic>T.\u00a0tricuspidata<\/jats:italic>\n                    . Further, purified\n                    <jats:italic>T.\u00a0tricuspidata<\/jats:italic>\n                    DNase showed a single sharp peak in reversed\u2010phase high\u2010performance\u00a0liquid chromatography\u00a0(RP\u2010HPLC) with an apparent molecular mass of 17\u2009kDa.\n                    <jats:italic>T.\u00a0tricuspidata<\/jats:italic>\n                    DNase exhibited potent DNA degrading activity performed using agarose gel electrophoresis, spectrophotometric assay, and DNA zymography. In addition, purified DNase from\n                    <jats:italic>T.\u00a0tricuspidata<\/jats:italic>\n                    was able to neutralize\n                    <jats:italic>E.\u00a0carinatus<\/jats:italic>\n                    venom\u2010induced mouse tail tissue necrosis and normalized elevated serum creatine kinase (CK) and lactate dehydrogenase (LDH) levels 30\u2009minutes post venom injection.\n                    <jats:italic>T.\u00a0tricuspidata<\/jats:italic>\n                    DNase was also able to reverse\n                    <jats:italic>E.\u00a0carinatus<\/jats:italic>\n                    venom\u2010induced histopathological changes and collagen depletion in mice tail tissue. All these observed pharmacological actions of\n                    <jats:italic>T.\u00a0tricuspidata<\/jats:italic>\n                    DNase were inhibited by sodium fluoride (NaF). This study provides scientific validation of the traditional use of\n                    <jats:italic>T.\u00a0tricuspidata<\/jats:italic>\n                    leaf paste in the healing of snakebite\u2010induced tissue necrosis and might be exploited to treat snake venom\u2010induced local toxicity.\n                  <\/jats:p>","DOI":"10.1002\/jcb.28115","type":"journal-article","created":{"date-parts":[[2018,12,2]],"date-time":"2018-12-02T23:32:09Z","timestamp":1543793529000},"page":"8319-8332","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Plant DNases are potent therapeutic agents against\n                    <i>Echis carinatus<\/i>\n                    venom\u2010induced tissue necrosis in mice"],"prefix":"10.1002","volume":"120","author":[{"given":"Gotravalli V.","family":"Rudresha","sequence":"first","affiliation":[{"name":"Department of Studies in Biochemistry University of Mysore, Manasagangothri Mysuru 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