{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:09:10Z","timestamp":1788944950353,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","award":["94\u201004\u201041\u201031178"],"award-info":[{"award-number":["94\u201004\u201041\u201031178"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005421","name":"Guilan University of Medical Sciences","doi-asserted-by":"publisher","award":["95012102"],"award-info":[{"award-number":["95012102"]}],"id":[{"id":"10.13039\/501100005421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Copy number variation (CNV) of DNA segments has been considered as an important component of genetic variation, affecting the quality and quantity of gene expression. Bone morphogenic protein 8A (BMP8A) has been reported to function in bone formation. With respect to the bone and joint complications in ankylosing spondylitis (AS), this investigation aimed to study the role of\n                      <jats:italic>BMP8A<\/jats:italic>\n                      gene CNV in impressing the gene expression as well as the disease risk.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 900 individuals, including 450 patients with AS and 450 healthy controls were enrolled. The copy numbers of\n                      <jats:italic>BMP8A<\/jats:italic>\n                      gene were detected by TaqMan real\u2010time polymerase chain reaction (PCR) method.\n                      <jats:italic>BMP8A<\/jats:italic>\n                      messenger RNA (mRNA) transcript level in peripheral blood mononuclear cells (PBMCs) was also measured by SYBR Green real\u2010time gene expression PCR method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      No significant association of\n                      <jats:italic>BMP8A<\/jats:italic>\n                      copy number was detected with the risk of AS.\n                      <jats:italic>BMP8A<\/jats:italic>\n                      mRNA expression level was significantly downregulated in patients compared with controls. mRNA expression level of\n                      <jats:italic>BMP8A<\/jats:italic>\n                      in both AS patients with and without syndesmophyte was significantly lower than the healthy control group. There was no correlation between the mRNA expression level of\n                      <jats:italic>BMP8A<\/jats:italic>\n                      and both demographic and clinical data of the patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Although\n                      <jats:italic>BMP8A<\/jats:italic>\n                      gene expression was downregulated in patients with AS, its copy number could not affect the transcript level of\n                      <jats:italic>BMP8A<\/jats:italic>\n                      gene in PBMCs and was not associated with susceptibility to AS in Iranian population. BMP8a may take into account as an indicator of bone formation process in AS, but it seems that mechanisms other than CNV may regulate this protein.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcb.28120","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T14:08:29Z","timestamp":1543414109000},"page":"8359-8365","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Association study of copy number variation in\n                    <i>BMP8A<\/i>\n                    gene with the risk of ankylosing spondylitis in Iranian population"],"prefix":"10.1002","volume":"120","author":[{"given":"Sara","family":"Shahba","sequence":"first","affiliation":[{"name":"Medical Biotechnology Research Center, School of Paramedicine, Guilan University of Medical Sciences Rasht Iran"},{"name":"Rheumatology Research Center, Tehran University of Medical Sciences Tehran 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