{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T09:08:49Z","timestamp":1788944929170,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,12,9]],"date-time":"2019-12-09T00:00:00Z","timestamp":1575849600000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2018,12,9]],"date-time":"2018-12-09T00:00:00Z","timestamp":1544313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2018,12,9]],"date-time":"2018-12-09T00:00:00Z","timestamp":1544313600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100011030","name":"Wind Energy Technologies Office","doi-asserted-by":"publisher","award":["DE\u2010AC05\u201006OR23100"],"award-info":[{"award-number":["DE\u2010AC05\u201006OR23100"]}],"id":[{"id":"10.13039\/100011030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dicentric chromosome assay (DCA) is routinely used for estimating the absorbed radiation dose in exposed humans. Optimal lymphocyte viability is crucial for reliable dose estimation and most cytogenetic laboratories prefer the receipt of blood samples within 24 to 36\u2009hours after collection. Delays in the shipment\/receipt of samples can occur sometimes under certain unforeseen circumstances: (1) Adverse weather conditions, (2) distant location of blood collection sites, and (3) shipping and handling of a large number of samples after radiological\/nuclear mass casualty incident(s). To circumvent some of these limitations, we evaluated the suitability of ex vivo irradiated blood samples stored in the presence of phytohemagglutinin (PHA) for 7 days at ambient temperature (22\u201024\u00b0C) for radiation biodosimetry. Blood samples stored in the presence of PHA for up to 7 days showed a higher mitotic index than blood samples stored without PHA. To verify the use of stored blood samples for DCA, frequencies of X\u2010rays induced dicentric chromosomes were analyzed in the blood samples that were cultured either 24 hours after exposure or 7 days later after storage. Our results indicate that storage of ex vivo irradiated blood samples in the presence of PHA at ambient temperature was found optimal for DCA and that the radiation doses estimated by dicentric chromosome frequencies were grossly similar between the fresh and stored blood samples. Our study suggests that reliable and accurate biodosimetry results can be obtained for triage using blood samples stored for up to a week at ambient temperature in the presence of PHA.<\/jats:p>","DOI":"10.1002\/jcb.28150","type":"journal-article","created":{"date-parts":[[2018,12,10]],"date-time":"2018-12-10T01:01:35Z","timestamp":1544403695000},"page":"8619-8629","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Extension of lymphocyte viability for radiation biodosimetry: Potential implications for radiological\/nuclear mass casualty incidents"],"prefix":"10.1002","volume":"120","author":[{"given":"Tammy","family":"Smith","sequence":"first","affiliation":[{"name":"Cytogenetics Biodosimetry Laboratory, Radiation Emergency Assistance Center and Training Site, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities Oak Ridge Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Escalona","sequence":"additional","affiliation":[{"name":"Cytogenetics Biodosimetry Laboratory, Radiation Emergency Assistance Center and Training Site, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities Oak Ridge Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terri","family":"Ryan","sequence":"additional","affiliation":[{"name":"Cytogenetics Biodosimetry Laboratory, Radiation Emergency Assistance Center and Training Site, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities Oak Ridge Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon K.","family":"Livingston","sequence":"additional","affiliation":[{"name":"Cytogenetics Biodosimetry Laboratory, Radiation Emergency Assistance Center and Training Site, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities Oak Ridge Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob T.","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Cellular and Molecular Biology University of Tennessee Knoxville Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9957-1513","authenticated-orcid":false,"given":"Adayabalam S.","family":"Balajee","sequence":"additional","affiliation":[{"name":"Cytogenetics Biodosimetry Laboratory, Radiation Emergency Assistance Center and Training Site, Oak Ridge Institute for Science and Education, Oak Ridge Associated Universities Oak Ridge Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,9]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.2307\/3571128"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01964820"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000802203630"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2009.11.001"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-26"},{"key":"e_1_2_8_1_7_1","first-page":"67","article-title":"Blood transport method for chromosome analysis of residents living near Semipalatinsk nuclear test site","volume":"58","author":"Rodzi M","year":"2009","journal-title":"Hiroshima J Med Sci"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jrr\/rrx049"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/rpd\/ncm137"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/16.2.115"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(87)90042-2"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.2203\/dose-response.13-060.Selvan"},{"key":"e_1_2_8_1_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gev072"},{"key":"e_1_2_8_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(99)00078-9"},{"key":"e_1_2_8_1_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000210152962"},{"key":"e_1_2_8_1_16_1","doi-asserted-by":"publisher","DOI":"10.2307\/3573852"},{"key":"e_1_2_8_1_17_1","first-page":"664","article-title":"Effect of storage duration on cell cycle kinetics and yield of chromosomal aberrations in PHA\u2010responsive human peripheral blood lymphocytes","volume":"30","author":"Jha AN","year":"1992","journal-title":"Indian J Exp Biol"},{"key":"e_1_2_8_1_18_1","first-page":"192","article-title":"Effects of storage of blood samples upon chromosome study","volume":"15","author":"Koresawa M","year":"1970","journal-title":"Jinrui Idengaku Zasshi"},{"key":"e_1_2_8_1_19_1","first-page":"258","article-title":"Influence of blood storage after in vitro exposure on the yield of micronuclei observed in human lymphocytes","volume":"169","author":"Lopez I","year":"1993","journal-title":"Strahlenther Onkol"},{"key":"e_1_2_8_1_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553008414550171"},{"key":"e_1_2_8_1_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553007714550591"},{"key":"e_1_2_8_1_22_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR1272.1"},{"key":"e_1_2_8_1_23_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR14974.1"}],"container-title":["Journal of Cellular Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28150","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcb.28150","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/jcb.28150","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcb.28150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:42:39Z","timestamp":1788943359000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcb.28150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,9]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1002\/jcb.28150"],"URL":"https:\/\/doi.org\/10.1002\/jcb.28150","archive":["Portico"],"relation":{},"ISSN":["0730-2312","1097-4644"],"issn-type":[{"value":"0730-2312","type":"print"},{"value":"1097-4644","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,9]]},"assertion":[{"value":"2018-10-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}