{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T07:57:51Z","timestamp":1770278271823,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T00:00:00Z","timestamp":1548374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010041","name":"Tianjin Science and Technology Committee","doi-asserted-by":"publisher","award":["12ZCZDSY03100"],"award-info":[{"award-number":["12ZCZDSY03100"]}],"id":[{"id":"10.13039\/501100010041","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Elevated plasma homocysteine (Hcy) concentration is considered as the diagnostic criteria of Hyperhomocysteinemia (HHcy), which is associated with the inflammatory response and blood\u2010brain barrier disruption. Previous studies have proposed that HHcy with hypertension was associated with the brain injury by enhancing the cerebrovascular permeability, however, the immune mechanism remains obscure. The purpose of the study is to explore the immunomodulatory mechanism of brain injury in spontaneously hypertensive rats (SHRs) induced by HHcy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Sixty SHRs were randomly assigned to three groups: SHR\u2010C (control group), SHR\u2010M (methionine group) and SHR\u2010T (treatment group). Physical examination of body weight, systolic blood pressure (SBP) and plasma Hcy content was measured every 4 weeks. Besides, T\u2010helper cell 17 and regulatory T cells (Treg)\u2010related inflammatory cytokines (interleukin [IL]\u20106, IL\u201017, IL\u201010, and transforming growth factor beta [TGF\u2010\u03b2]) and genes (ROR\u03b3t and FoxP3) were detected by enzyme\u2010linked immunosorbent assay, quantitative polymerase chain reaction , Western blot, and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High methionine diet could cause weight loss, SBP rising, and plasma Hcy content significantly elevated. IL\u201016 and IL\u201017A levels in peripheral blood and in brain tissue both lifted, while IL\u201010 and TGF\u2010\u03b2 levels dropped; ROR\u03b3t expression raised in brain, nevertheless, FoxP3 levels were the opposite. After the intervention with vitamin B6, B12, and folic acid in SHR\u2010T group, these trends would be eased or completely changed. Furthermore, brain tissue slices showed that IL\u201017\u2010positive cells tended to decrease, and IL\u201010\u2010positive cells increased in SHR\u2010T group, which was reversed in SHR\u2010M group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HHcy may promote inflammation that can lead to brain lesions and down\u2010regulate immune response to protect the brain.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.28217","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T11:27:00Z","timestamp":1548415620000},"page":"9421-9429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The immunomodulatory mechanism of brain injury induced by hyperhomocysteinemia in spontaneously hypertensive rats"],"prefix":"10.1002","volume":"120","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Geriatric the Second Hospital of 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