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And reported compounds encourage this hypothesis greatly based on the remarkable anti\u2010AD effects in vivo. To illustrate the mechanism in detail, the actions of a selected QC inhibitor (<jats:bold>23<\/jats:bold>) were assessed firstly in a cell system here. It was demonstrated that QC activities and the generation of pyroglutamate\u2010modified \u03b2\u2010amyloids in PC12 cells were both inhibited obviously after the treatment of <jats:bold>23<\/jats:bold>. A total of 13 and 15 genes were up\u2010 and downregulated significantly in treated cells by RNA\u2010sequencing analysis. Quantitative real\u2010time polymerase chain reaction, enzyme\u2010linked immunosorbent assay, WB, and immuno\ufb02uorescence analysis supported the effects of <jats:bold>23<\/jats:bold> on the transcriptome of PC12 cells consequently. The expressions of chaperones, heat shock proteins (HSP) 70, and 90, were upreglutated, while gene expression of actin and the level of encoded protein were reduced significantly in PC12 cells with the treatment. Furthermore, the regulations of ribosome were observed after the treatment. These results indicate the potency of <jats:bold>23<\/jats:bold> to improve the translation, expression and folding regulation of proteins and affect the multivalent cross\u2010linking of cytoskeletal protein and other proteins subsequently in the cell system and might contribute to the understanding of the mechanism of QC inhibitor as potential anti\u2010AD agents.<\/jats:p>","DOI":"10.1002\/jcb.28222","type":"journal-article","created":{"date-parts":[[2018,12,24]],"date-time":"2018-12-24T07:23:56Z","timestamp":1545636236000},"page":"9460-9471","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Glutaminyl cyclase inhibitor contributes to the regulation of HSP70, HSP90, actin, and ribosome on gene and protein levels in vitro"],"prefix":"10.1002","volume":"120","author":[{"given":"Xi","family":"Yu","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences, Health Science Center, Shenzhen University 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