{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:15:52Z","timestamp":1760300152430,"version":"3.40.4"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T00:00:00Z","timestamp":1549324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gluconeogenesis (GN) is increased in patients with cancer cachexia, but is reduced in liver perfusion of Walker\u2010256 tumor\u2010bearing cachectic rats (TB rats). The causes of these differences are unknown. We investigated the influence of circulating concentrations of lactate (NADH generator) and NADH on GN in perfused livers of TB rats. Lactate, at concentrations similar to those found on days 5 (3.0\u2009mM), 8 (5.5\u2009mM), and 12 (8.0\u2009mM) of the tumor, prevented the reduction of GN from 2.0\u2009mM lactate (lactatemia of healthy rat) in TB rats. NADH, 50 or 75\u2009\u03bcM, but not 25\u2009\u03bcM, increased GN from 2.0\u2009mM lactate in TB rats to higher values than healthy rats. High concentrations of pyruvate (no NADH generator, 5.0 and 8.0\u2009mM) did not prevent the reduction of GN from 2.0\u2009mM pyruvate in TB rats. However, 50 or 75\u2009\u03bcM NADH, but not 25\u2009\u03bcM, increased GN from 2.0\u2009mM pyruvate in TB rats to similar or higher values than healthy rats. High concentration of glutamine (NADH generator, 2.5\u2009mM) or 50\u2009\u03bcM NADH prevented the reduction of GN from 1\u2009mM glutamine in TB rats. Intraperitoneal administration of pyruvate (1.0\u2009mg\/kg) or glutamine (0.5\u2009mg\/kg) similarly increased the glycemia of healthy and TB rats. In conclusion, high lactate concentration, similar to hyperlactatemia, prevented the reduction of GN in perfused livers of TB rats, an effect probably caused by the increased redox potential (NADH\/NAD<jats:sup>+<\/jats:sup>). Thus, the decreased GN in livers from TB rats is due, at least in part, to the absence of simulation of in vivo hyperlactatemia in liver perfusion studies.<\/jats:p>","DOI":"10.1002\/jcb.28384","type":"journal-article","created":{"date-parts":[[2019,2,5]],"date-time":"2019-02-05T12:27:04Z","timestamp":1549369624000},"page":"11068-11080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Infusion of high concentration of lactate in perfused liver, simulating in vivo hyperlactatemia, prevents the reduction of gluconeogenesis in Walker\u2010256 tumor\u2010bearing rats"],"prefix":"10.1002","volume":"120","author":[{"given":"Isabele Gon\u00e7alves","family":"Frasson\u2010Uemura","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuliana Regina","family":"Biazi","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele Romani","family":"Miksza","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Campos Lima","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila","family":"Cassolla","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisele 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