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A recent study reported that miRNAs may be important transcriptional regulators. In this study, we found increased expression of miR\u2010939\u20105p and decreased expression of CD2AP in the peripheral blood of patients with NS. However, miR\u2010939\u20105p did not show a regulatory effect on the 3\u2032\u2010untranslated region of CD2AP. The expression levels of specific protein 1 and adenovirus E2 promoter\u2010binding factor 1, important transcription regulators in the promoter region of CD2AP, were also not affected by microRNA (miR)\u2010939\u20105p. We confirmed that miR\u2010939\u20105p is in the nucleus by fluorescent in situ hybridization and cytoplasmic separation polymerase chain reaction. The promoter plasmid and miR\u2010939\u20105p were cotransfected into HEK\u2010293 cells, and the luciferase reporter gene assay was used to analyze the promoter activity. We found that miR\u2010939\u20105p binds to a specific sequence in the CD2AP promoter. miR\u2010939\u20105p was confirmed to reduce the recruitment of RNA polymerase II to the CD2AP promoter region by chromatin immunoprecipitation. These findings improve our understanding of the mechanism of miR\u2010939\u20105p in NS and provide potential molecular therapeutic targets for NS.<\/jats:p>","DOI":"10.1002\/jcb.28413","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T09:26:27Z","timestamp":1550049987000},"page":"11366-11374","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["miR\u2010939\u20105p decreases the enrichment of RNA polymerase II in the promoter region of CD2AP involved in nephrotic syndrome"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6186-0114","authenticated-orcid":false,"given":"Jin\u2010Ya","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatrics, the First Affiliated Hospital Nanjing Medical University Nanjing Jiangsu Province 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