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Therefore, this study aims to investigate functions of the miR\u2010154, which is well\u2010documented in a series of cell activities, IDD, and other relevant mechanisms. Lumbar nucleus pulposus (NP) samples were collected from patients with IDD and the control group. After solexa sequencing and bioinformatical analysis, the results showed that miR\u2010154 was increased in NP cells of patients with IDD. Inhibition of miR\u2010154 increased type II collagen and aggrecan and decreased mRNA expressions of collagenase\u20103 (MMP13) and aggrecanase\u20101 (ADAMTS4), whereas overexpression of miR\u2010154 reversed such effects in NP cells. In addition, the luciferase reporter assay revealed that fibroblast growth factor 14 (FGF14) is a direct target of miR\u2010154 and that the overexpression of FGF14 leads to similar effects as inhibition of miR\u2010154 did. In conclusion, the results suggested that miR\u2010154 participates in the development of IDD and its effects are mediated via targeting FGF14. Thus, miR\u2010154 may be thought as a potential etiological factor for IDD and may provide insights into a therapeutic target to treat IDD.<\/jats:p>","DOI":"10.1002\/jcb.28471","type":"journal-article","created":{"date-parts":[[2019,3,2]],"date-time":"2019-03-02T07:49:55Z","timestamp":1551512995000},"page":"11900-11907","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Upregulated miR\u2010154 promotes ECM degradation in intervertebral disc degeneration"],"prefix":"10.1002","volume":"120","author":[{"given":"Jingjie","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Qilu Hospital, Shandong University Jinan China"},{"name":"Department of Spine Yantaishan Hospital Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Yuhuangding Hospital Yantai 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