{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T05:20:43Z","timestamp":1772083243968,"version":"3.50.1"},"reference-count":57,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Basic and Applied Research Support Grant Project","award":["15089"],"award-info":[{"award-number":["15089"]}]},{"name":"Capacity Building Grant Project","award":["4940"],"award-info":[{"award-number":["4940"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>microRNAs (miRNAs) are implicated in carcinogenesis and their expression in biological fluids offer great potential as nucleic acid markers for cancer detection and progression. Authors investigated the expression level of miRNAs (miRNA\u201021, miRNA\u2010126, and miRNA\u2010155) to evaluate their role as diagnostic and prognostic markers for breast cancer compared with other commonly used protein\u2010based markers (CEA and CA15\u20103). Serum samples from patients with breast cancer (n\u2009=\u200996), patients with benign breast lesion (n\u2009=\u200947), and healthy individuals (n\u2009=\u200939) were enrolled for detection of miRNA expression levels and protein\u2010based tumor markers using fluorescent real\u2010time quantitative polymerase chain reaction and enzyme\u2010linked immunosorbent assay, respectively. Correlation among investigated markers with clinicopathological factors and clinical outcomes were determined. Expression of miRNA\u201021 and miRNA\u2010155 revealed significant increases in patients with breast cancer compared with both benign and control groups, the same result was reported for tumor markers; on the other hand, miRNA\u2010126 was significantly decreased in breast cancer group as compared with the other two groups. miRNA frequencies were significantly related to clinical staging and histological grading as compared with tumor markers. Patients with breast cancer with increased miRNA\u201021 and miRNA\u2010155 and decreased miRNA\u2010126 expressions had significantly worse disease\u2010free survival, while only miRNA\u201021 and miRNA\u2010126 showed poor OS (<jats:italic>P<\/jats:italic>&lt;\u20090.005). In conclusion, investigated miRNAs were superior over tumor markers for the early stage of breast cancer especially those with high\u2010risk factor and their assessment in blood facilitates their role as a potential prognostic molecular marker.<\/jats:p>","DOI":"10.1002\/jcb.28496","type":"journal-article","created":{"date-parts":[[2019,3,2]],"date-time":"2019-03-02T07:43:54Z","timestamp":1551512634000},"page":"12321-12330","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Clinical significance of blood\u2010based miRNAs as diagnostic and prognostic nucleic acid markers in breast cancer: Comparative to conventional tumor markers"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7104-7194","authenticated-orcid":false,"given":"Menha","family":"Swellam","sequence":"first","affiliation":[{"name":"Biochemistry Department Genetic Engineering and Biotechnology Research Division National Research Centre Giza Egypt"},{"name":"High Throughput Molecular and Genetic Laboratory Center for Excellence for Advanced Sciences, National Research Centre Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9158-9559","authenticated-orcid":false,"given":"Amal","family":"Ramadan","sequence":"additional","affiliation":[{"name":"Biochemistry Department Genetic Engineering and Biotechnology Research Division National Research Centre Giza Egypt"},{"name":"High Throughput Molecular and Genetic Laboratory Center for Excellence for Advanced Sciences, National Research Centre Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enas A.","family":"El\u2010Hussieny","sequence":"additional","affiliation":[{"name":"Zoology Department Faculty of Science, Ain Shams University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0485-7138","authenticated-orcid":false,"given":"Noha M.","family":"Bakr","sequence":"additional","affiliation":[{"name":"Biochemistry Department Genetic Engineering and Biotechnology Research Division National Research Centre Giza Egypt"},{"name":"High Throughput Molecular and Genetic Laboratory Center for Excellence for Advanced Sciences, National Research Centre Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naglaa M.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Clinical Pathology Department National Cancer Institute, Cairo University Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Emam","family":"Sobeih","sequence":"additional","affiliation":[{"name":"Medical Oncology Department National Cancer Institute, Cairo University Cairo 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