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Podocyte injury exerts a crucial effect on DN pathogenesis. MicroRNA\u2010503 (miR\u2010503) has been reported in various diseases including DN. Here, we investigated the detailed mechanism of miR\u2010503 in the podocyte injury of DN. The functional role of miR\u2010503 was investigated in cultured podocytes and diabetic rats. Podocyte injury was evaluated by migration and apoptosis experiments in podocytes and we observed that high glucose elevated miR\u2010503 in a time and dose\u2010dependent manner. Meanwhile, E2F transcription factor 3 (E2F3), as a crucial regulator in multiple diseases, was predicted as a potential target of miR\u2010503 here. It was shown that E2F3 was greatly decreased in podocytes incubated with high glucose and miR\u2010503 modulated its expression negatively. In addition, downregulation of E2F3 contributed to podocyte injury, which was reversed by miR\u2010503 inhibitors in vitro. Furthermore, we proved that increase of miR\u2010503 resulted in an unfavorable renal function in diabetic rats via targeting E2F3. These revealed for the first time that the overexpression of miR\u2010503 promoted podocyte injury via targeting E2F3 in diabetic nephropathy and miR\u2010503\/E2F3 axis might represent a pathological mechanism of diabetic nephropathy progression.<\/jats:p>","DOI":"10.1002\/jcb.28524","type":"journal-article","created":{"date-parts":[[2019,3,5]],"date-time":"2019-03-05T05:30:33Z","timestamp":1551763833000},"page":"12574-12581","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["MicroRNA\u2010503 contributes to podocyte injury via targeting E2F3 in diabetic nephropathy"],"prefix":"10.1002","volume":"120","author":[{"given":"Fangfang","family":"Zha","sequence":"first","affiliation":[{"name":"Department of Nephrology Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Qingpu District Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Nephrology Huai\u2019an Second People\u2019s Hospital, The Affiliated Hospital of Xuzhou Medical University Xuzhou Huai\u2019an P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Qingpu District Shanghai P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nephrology Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Qingpu District Shanghai P. R. 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