{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T13:26:05Z","timestamp":1742390765202,"version":"3.37.3"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701453","81601307"],"award-info":[{"award-number":["81701453","81601307"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2016HL05"],"award-info":[{"award-number":["ZR2016HL05"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To explain long noncoding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) affects the mechanism in development of pre\u2010eclampsia. The pathological changes of normal, mild, and severe pre\u2010eclampsia were evaluated by hematoxylin and eosin staining and measured the lncRNA GHET1 expression in different tissues by reverse\u2010transcription polymerase chain reaction. In the cell experiment, the BeWo cells were randomly divided into three groups: normal control (NC) group, model group, and lncRNA group. The JEG3 cells of the model and lncRNA groups were cultured in the hypoxia condition. The JEG3 cells invasion and migration abilities were measured by Tanswell and wound\u2010healing assays. The relative protein expressions of different groups were evaluated by Western blot (WB) assay. Compared with normal puerperal, the lncRNA GHET1 gene expression of pre\u2010eclampsia was significantly downregulated (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). In the cell experiment, the invasion cell number and wound\u2010healing rate of the model group were significantly suppressed compared with the NC group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). However, the invasion cell number and wound\u2010healing rate of lncRNA group were enhanced by lncRNA GHET1 overexpression (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). In WB assay, the E\u2010cadherin, fibronectin, and vimentin proteins expression showed significant differences between the model and lncRNA groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). lncRNA GHET1 overexpression had restored cell invasion and migration abilities reduced by hypoxia in pre\u2010eclampsia.<\/jats:p>","DOI":"10.1002\/jcb.28531","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T22:11:47Z","timestamp":1552428707000},"page":"12647-12652","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["lncRNA GHET1 has effects in development of pre\u2010eclampsia"],"prefix":"10.1002","volume":"120","author":[{"given":"Shuhong","family":"Li","sequence":"first","affiliation":[{"name":"Departments of Obstetrics and Gynecology The Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Liu","sequence":"additional","affiliation":[{"name":"Departments of Obstetrics and Gynecology The Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanqiang","family":"Kong","sequence":"additional","affiliation":[{"name":"Departments of Emergency The Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanli","family":"Wang","sequence":"additional","affiliation":[{"name":"Departments of Obstetrics and Gynecology The Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2940-4696","authenticated-orcid":false,"given":"Ning","family":"Li","sequence":"additional","affiliation":[{"name":"Departments of Central Laboratory The Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanfen","family":"Zou","sequence":"additional","affiliation":[{"name":"Departments of Obstetrics and Gynecology The Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai People's Republic of 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