{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T19:35:22Z","timestamp":1781811322505,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences and Health Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Prenatal white matter injury is a serious problem due to maternal inflammation leading to postnatal disabilities. In this study, we used the periventricular leukomalacia (PVL) model as a common prenatal white matter injury by maternal administration of lipopolysaccharide (LPS). Neural stem cells (NSCs) have shown therapeutic ability in neurological disorders through a different mechanism such as immunomodulation. Here, we studied the preventive potential of NSCs following in utero transplantation into the embryonic lateral ventricle in an LPS\u2010induced white matter injury model. Pregnant animals were divided into three groups and received phosphate buffered saline, LPS, or LPS\u2009+\u2009NSCs. The brains of offspring were obtained and evaluated by real\u2010time polymerase chain reaction (PCR), immunohistochemy, enzyme\u2010linked immunosorbent assay (ELISA), terminal deoxynucleotidyl transferase\u2010mediated biotinylated\u2010dUTP nick\u2010end labeling (TUNEL), and caspase\u20103 activity assay. The LPS\u2010induced maternal inflammation degenerated the myelin sheath in the offspring periventricular region which was associated with an increased microglial number, oligodendrocytes degeneration, proinflammatory cytokine secretion, and cell apoptosis. The transplanted NSCs homed into the brain and ameliorated the evaluated parameters. The expression of proinflammatory cytokines interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u20106, and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), cell apoptosis and caspase\u20103 activity were inhibited by NSCs. In addition, Olig2 and myelin basic protein immunohistochemy staining showed that prenatal NSCs transplantation augmented the myelination in the periventricular white matter of offspring. In conclusion, we think that prenatal therapeutic strategies, such as in utero NSCs transplantation, may prevent prenatal white matter injury after birth.<\/jats:p>","DOI":"10.1002\/jcb.28548","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T20:57:42Z","timestamp":1552424262000},"page":"12785-12795","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["In utero transplantation of neural stem cells ameliorates maternal inflammation\u2010induced prenatal white matter injury"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0308-2948","authenticated-orcid":false,"given":"Maryam","family":"Borhani\u2010Haghighi","sequence":"first","affiliation":[{"name":"Department of Anatomy School of Medicine, Tehran University of Medical Sciences Tehran 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