{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:37:49Z","timestamp":1791927469875,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T00:00:00Z","timestamp":1552953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Health Care of Yellow Crane Talent Plan","award":["Project NO. 17"],"award-info":[{"award-number":["Project NO. 17"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoporosis (OP) results from the impaired function of endogenous bone marrow mesenchymal stem cells (BMSCs). Icariin (ICA) has shown potential osteoprotective effects. However, the molecular mechanism for the anabolic action of ICA remains largely unknown. The objective\u00a0of the present study is\u00a0to investigate whether ICA prevents bone loss by acting on BMSCs via affecting the level of autophagy after ovariectomy (OVX). The BMSCs were extracted from BALB\/c mice treated with ICA, chloroquine (CQ, an autophagy inhibitor) or ICA\u2009+\u2009CQ. The OVX mice were injected with ICA, CQ, or ICA\u2009+\u2009CQ for 1 month. We performed Alizarin Red staining and alkaline phosphatase staining to detect osteogenic differentiation of BMSCs. Micro\u2010CT, hematoxylin and eosin staining, Oil Red O staining, and tartrate\u2010resistant acid phosphatase staining were used to assess the bone mass, lipid droplets and osteoclasts in femurs. Autophagy activity in BMSCs from different groups was evaluated by Western blot analysis. The osteogenic differentiation of BMSCs from OVX\u2010induced OP mice was decreased. Treatment with ICA reduced bone loss and formation of osteoclasts and increased osteogenic differentiation of BMSCs in vitro and vivo. In addition, autophagy was enhanced in BMSCs of OVX mice treated with ICA. Our results indicate that ICA prevents OVX\u2010induced bone loss possibly by strengthening the osteogenic differentiation of BMSCs via increasing autophagic activity.<\/jats:p>","DOI":"10.1002\/jcb.28585","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T00:53:09Z","timestamp":1553043189000},"page":"13121-13132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Icariin promotes osteogenic differentiation of bone marrow stromal cells and prevents bone loss in OVX mice via activating autophagy"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4998-3074","authenticated-orcid":false,"given":"Xiaoxiao","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Zhongnan Hospital of Wuhan University Wuhan 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