{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T23:23:43Z","timestamp":1778282623669,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To study the effect of miRNA\u2010200b on hepatic fibrosis induced by CCl<jats:sub>4<\/jats:sub> in mice. The C59BL\/6 mice were randomly divided into three groups (normal control [NC], CCLR model [Model], and CCl\n<jats:sub>4<\/jats:sub>\u2009+\u2009miRNA\u2010200b [miRNA]). The hepatic fibrosis was induced by CCl\n<jats:sub>4<\/jats:sub> injected subcutaneously twice per week in Model and miRNA groups. After 6 weeks building model, the mice of miRNA group were injected the miRNA\u2010200b from caudal vein twice per week. The mice of Model and miRNA groups were continuously fed for 3 weeks. The IL\u20101\u03b2, IL\u20106, and TNF\u2010\u03b1 concentrations of serum were measured by enzyme\u2010linked immunosorbent assay. The hepatic tissues of difference groups were observed by hematoxylin and eosin (H&amp;E) staining, sirius red staining, Masson staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay and measured toll\u2010like receptor 4 (TLR4) and nuclear factor\u2010\u03baB (NF\u2010\u03baB) proteins expressions by western blot assay. The correlation between miRNA\u2010200b and TLR4 were analyzed by dual luciferase target assay. Compared with NC group, the interleukin\u20101\u03b2 (IL\u20101\u03b2), IL\u20106, and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) concentrations of Model group were significantly upregulated (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). With miRNA\u2010200b overexpression, the IL\u20101\u03b2, IL\u20106, and TNF\u2010\u03b1 concentrations were significantly suppressed (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). The pathologies were improved by H&amp;E staining, sirius red staining, and Masson staining; meanwhile, the hepatic cell apoptosis rate was significantly suppressed (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The TLR4 and NF\u2010\u03baB protein expressions of miRNA group were significantly suppressed compared with the Model group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05, respectively). By dual luciferase target assay, the TLR4 was a target gene of miRNA\u2010200b. The miRNA\u2010200b upregulation improved hepatic fibrosis induced by CCl\n<jats:sub>4<\/jats:sub> via regulation of TLR4 in vivo.<\/jats:p>","DOI":"10.1002\/jcb.28599","type":"journal-article","created":{"date-parts":[[2019,3,29]],"date-time":"2019-03-29T04:20:10Z","timestamp":1553833210000},"page":"13254-13261","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["RETRACTED: miRNA\u2010200b improves hepatic fibrosis induced by CCL<sub>4<\/sub> by regulating toll\u2010like receptor 4 in mice"],"prefix":"10.1002","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6803-4336","authenticated-orcid":false,"given":"Yan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases Binzhou Central Hospital of Shandong Province Binzhou Shandong 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