{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:14:40Z","timestamp":1791360880093,"version":"4.3.1"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T00:00:00Z","timestamp":1553644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2015A030310504"],"award-info":[{"award-number":["2015A030310504"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Impaired trabecular meshwork (TM) outflow is implicated in the pathogenesis of primary open\u2010angle glaucoma (POAG). We previously identified the association of a caveolin\u20101 (<jats:italic>CAV1<\/jats:italic>) variant with POAG by genome\u2010wide association study. Here we report a study of \n<jats:italic>CAV1<\/jats:italic> knockout (KO) effect on human TM cell properties. We generated human \n<jats:italic>CAV1<\/jats:italic>\u2010KO TM cells by CRISPR\/Cas9 technology, and we found that the \n<jats:italic>CAV1<\/jats:italic>\u2010KO TM cells less adhered to the surface coating than the wildtype TM cells by 69.34% (\n<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), but showed no difference in apoptosis. Higher endocytosis ability of dextran and transferrin was also observed in the \n<jats:italic>CAV1<\/jats:italic>\u2010KO TM cells (4.37 and 1.89\u2010fold respectively, \n<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), compared to the wildtype TM cells. Moreover, the \n<jats:italic>CAV1<\/jats:italic>\u2010KO TM cells had higher expression of extracellular matrix\u2010degrading enzyme genes (\n<jats:italic>ADMTS13<\/jats:italic> and \n<jats:italic>MMP14<\/jats:italic>) as well as autophagy\u2010related genes (\n<jats:italic>ATG7<\/jats:italic> and \n<jats:italic>BECN1<\/jats:italic>) and protein (LC3B\u2010II) than the wildtype TM cells. In summary, results from this study showed that the \n<jats:italic>CAV1<\/jats:italic>\u2010KO TM cells have reduced adhesion with higher extracellular matrix\u2010degrading enzyme expression, but increased endocytosis and autophagy activities, indicating that \n<jats:italic>CAV1<\/jats:italic> could be involved in the regulation of adhesion, endocytosis, and autophagy in human TM cells.<\/jats:p>","DOI":"10.1002\/jcb.28613","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:35:52Z","timestamp":1553733352000},"page":"13382-13391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Caveolin\u20101 regulates human trabecular meshwork cell adhesion, endocytosis, and autophagy"],"prefix":"10.1002","volume":"120","author":[{"given":"Zhenggen","family":"Wu","sequence":"first","affiliation":[{"name":"Joint Shantou International Eye Center of Shantou University and the Chinese University 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