{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:17:48Z","timestamp":1740104268115,"version":"3.37.3"},"reference-count":34,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T00:00:00Z","timestamp":1553904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>High association between microRNA\u2010497 (miR\u2010497) inhibition and the improvement of myocardial infarction (MI) has been proved. Bone marrow mesenchymal stem cells (BMSCs) therapy is regarded as a highly promising approach to MI treatment. We studied the functional role of miR\u2010497 inhibition in the transplantation of BMSCs for MI treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BMSCs were isolated from 10 to 14 days old male Sprague\u2010Dawley (SD) rats for in vitro and in vivo experiments. First, flow cytometry was used for BMSCs identification. miR\u2010497 antagomir and agomir were transfected into BMSCs, and the migratory capacity was detected by wound healing assay. Protein levels were analyzed by Western blot analysis. Second, rat MI models were constructed and injected with each experimental group BMSCs. Four weeks later, the cellular morphology of cardiomyocyte and infarcted size was observed after histopathologic evaluation (HE) and Masson\u2019s trichrome staining. Moreover, WNT3A siRNA (siWNT3A) was used for further investigating the involvement of Wnt\/\u03b2\u2010catenin pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>BMSCs were confirmed to be CD90+ CD45\u2212 CD11b\/c\u2212 cells. The number of rats with wound closure increased more in miR\u2010497 inhibitor group than that in agomir group, the number markedly decreased in agomir group (\n<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). As the miR\u2010497 decreased, the protein levels of WNT3A, matrix metalloproteinase\u20109 and \u03b2\u2010catenin were notably increased. The injection of BMSCs inhibiting miR\u2010497 repaired almost all infarcted zones. siWNT3A, on the contrary, could decrease the wound closure rate and relative protein levels and inhibit MI treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>miR\u2010497 antagomir contributes to BMSCs transplantation for MI treatment by Wnt\/\u03b2\u2010catenin activation, and Wnt\/\u03b2\u2010catenin pathway is essential for the functional effects of miR\u2010497 antagomir.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcb.28615","type":"journal-article","created":{"date-parts":[[2019,3,30]],"date-time":"2019-03-30T09:54:09Z","timestamp":1553939649000},"page":"13403-13412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["microRNA\u2010497 inhibition mitigates myocardial infarction via enhancing wingless\/integrated signal pathway in bone marrow mesenchymal stem cells"],"prefix":"10.1002","volume":"120","author":[{"given":"Yu","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Cardiology Jiangxi Provincial People\u2019s Hospital Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7825-9280","authenticated-orcid":false,"given":"Zhiying","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Cardiology The Fourth Affiliated Hospital of Nanchang University Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Jiangxi Provincial People\u2019s Hospital Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Jiangxi Provincial People\u2019s Hospital Nanchang Jiangxi 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